摘要
建立了一种使用岛津超高效液相色谱仪LC-30A测定粮食中玉米赤霉烯酮的方法。实验结果表明：在浓度10-500μg／L内，校准曲线相关系数为0．9998；进样量2μL，仪器检出限为1．5μg／L，定量限为5μg／L。3个浓度下标准品的保留时间和峰面积的相对标准偏差分别在0．03％～0．15％和0．28％～1．71％；玉米样品平均加标回收率为92．7％-104．0％。
A method was established for determining zearalenone in grain by high performance liquid chromatography. The linear detection ranges of zearalenone were 10-500μg/L with correlation coefficients of 0.999. The retention volume of zearalenone was 2μL,the detection limit was 1.5 μg/L, and the limit of quantification was 5μg/L. The relative standard deviations of retention time and peak area under three concentrations were between 0.03% to 0.15% and 0.28% to 1.71%. Recovery rates in corn spiked with zearalenone were between 85% to 102%.
出处
《粮食科技与经济》
2015年第5期40-41,56,共3页
Food Science And Technology And Economy
基金
北京市科学技术委员会项目"粮食及其制品中真菌毒素快速检测技术研究与应用"(Z121100008512001)
关键词
玉米赤霉烯酮
粮食
超高效液相色谱
Zearalenone, grain, high performance liquid chromatography
