摘要
目的研究新训期间维生素E联合按摩对徒步行军后骨骼肌的保护作用及其保护机制。方法选取平素身体健康且无正规体育训练史的60名男性新兵随机分为A组(空白对照组)、B组(维生素E组)、C组(维生素E联合按摩组),每组均为20名,其中B、C组连续7 d服用维生素E(4 mg·kg-1·d-1),A组给予相同体积的橄榄油,7 d后让60名新兵进行90 km(3 d走完)徒步行军,行军过程中C组给予腿部按摩(20 min/次,2/d),A、B两组不做任何处理,徒步行军结束后2 h、3 h、6 h、24 h测量外周血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、活性氧类物质(ROS)、丙二醛(MDA)浓度的变化。结果 A组外周血清CK、LDH、ROS、MDA值各个时间点均高于其他两组,而GSH-PX、SOD活性值各个时间点均低于其他两组,差异均有统计学意义(P<0.05);B组外周血清CK、LDH、ROS、MDA值各个时间点均高于C组,而GSH-PX、SOD活性值各个时间点均低于C组,差异均有统计学意义(P<0.05)。三组的外周血清GSH-PX、SOD活性均于行军后开始下降,6 h达到最低值,24 h开始升高;三组新兵外周血ROS、MDA均于行军后开始升高,行军结束后6 h达到高峰,行军结束后24 h开始下降。结论维生素E联合按摩能通过调节氧化与抗氧化平衡来抑制长时间运动中所导致的肌肉损伤。
Objective To observe the effect of Vitamin E combined with massage on skeletal muscle injury caused by march. Methods Sixty new recruits who was not formally disciplined were randomly divided into three groups （every group include 20 recruits）, group A （control group）, group B （given Vitamin E）, group C （given Vitamin E and massage）. New recruits in group B and group C took Vitamin E （4 mg·kg-1·d-1） for 7 days, while those in group A were given olive oil. Then the recruits marched 90 km for 3 days. The recruits in group C were given massage （20 min one time, twice a day） in leg. Creatine kinase （CK）, lactate dehydrogenase （LDH）, Glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD）, reactive oxygen species （ROS）, malondialdehyde （MDA） in the three groups were detected 2 h, 3 h, 6 h and 24 h after march. Results The activity of CK, LDH, ROS and the concentration of MDA in group A all time points were higher than those in other two groups after march （P＆lt;0.05）, while the GSHG-PX, SOD at all time points were lower than those in other two groups after march （P〈0.05）. The activity of CK, LDH, ROS and the concentration of MDA in group B at all time points were higher than those in group C （P〈0.05）, while the GSHG-PX, SOD at all time points were lower than those in group C at all time points （P〈0.05）. The activity of GSH-PX, SOD were reduced after march, reached the bottom at 6 h, and then increased at 24 h in the three groups. The activity of ROS, LDH, ROS and the concentration of MDA were increased after march, reached the peak at 6 h after march, and decreased at 24 h after march in the three groups. Conclusion Vitamin E combined with massage can inhibit muscle injury during long movement through balancing oxidation and antioxidation.
出处
《海南医学》
CAS
2015年第20期2970-2973,共4页
Hainan Medical Journal
基金
国家社会科学基金青年项目(编号:11CTY023)
