摘要
目的比较两种不同方法提取冻存全血中基因组DNA的效果和特点。方法分别用改良碘化钾法和Promega全血基因组提取试剂盒提取全血基因组DNA,通过紫外分光光度仪、凝胶电泳、PCR进行检测。结果改良碘化钾法和试剂盒法提取的基因组DNA浓度分别为(330.9±0.94)ng/μL和(490.3±3.16)ng/μL;纯度为(1.87±0.03)和(1.85±0.06)。试剂盒法提取的基因组DNA含量稍高于改良碘化钾法,质量和纯度无显著差异。结论试剂盒法更简便、快速、无毒地进行提取DNA,但价格较贵;改良碘化钾法价格低廉,适合大量临床血液标本的提取,能够满足分子生物学实验的需要。
Objective To compare the effect and characteristics of genomic DNA extraction from frozen whole blood using two different methods. Methods Genomic DNA were extracted with potassium iodide method and Promega Genomic DNA PtLrification Kit.The detection was performed by UV spectrophotometry, gel electrophoresis and PCR. Results The concentration of genomic DNA extracted by potassium iodide method and Promega Genomic DNA Purification Kit method was： （330.9±0.94）ng/μL and （490.3±3.16）ng/pL; and the ratio of A260/A280 was： （1.87±0.03） and （1.85±0.06）.The quantity of genomic DNA extracted by kit is slightly higher than that by potassium iodide method. There is no significant difference in DNA quality and purity between two methods. Conclusion Kit is the more simple, faster, non-toxic method compared with the potassium iodide method, but the cost is expensive. Potassium iodide method is helpful to extract genomic DNA from a large number of clinical blood samples due to the low cost. Two methods are able to meet the needs of molecular biology experiments.
出处
《中国医药指南》
2015年第30期16-17,共2页
Guide of China Medicine
关键词
基因组DNA
碘化钾法
改良法
外周血
Genomic DNA
Potassium iodide method
Improved method
Peripheral blood
