摘要
以杜香为原料,采取水提醇沉法提取杜香多糖,利用红外和紫外分析,发现提取物含多糖典型基团,粗提物中多糖含量为69.95%。通过测定多糖对DPPH自由基（DPPH·）、超氧阴离子自由基（·O-2）、羟基自由基（·OH）的清除能力、总还原能力,得到杜香多糖清除DPPH·、·O-2、·OH的EC50值分别为0.52,1.41,1.71mg/mL,还原力接近BHT且与浓度呈正相关,杜香多糖具有较强的抗氧化活性。杜香多糖在190℃以内稳定,在190~550℃范围内发生热解,其结晶度为3.6%,结晶度低,分子刚性差。
The polysaccharide was extracted from Ledum,and the content and characteristics of polysaccharide fromLedum were investigated by IR,UV spectra,thermal analysis（TG）and X-ray diffraction（XRD）.The results from IR and UV spectra showed that the extracts containing the typical group of polysaccharide.It was noted that the content of Ledumpolysaccharide was 69.95%.In addition,the antioxidant activity containing total reductive ability,scavenging ability for DPPH radical（DPPH·）,hydroxyl radical（·OH）and superoxide anion radical（· O-2）were investigated.The results showed that the EC50 for scavenging DPPH·,·O-2,·OH were0.52,1.41,1.71 mg/mL,which means that the polysaccharide fromLedumpossessed a notable antioxidant activity.Moreover,the antioxidant ability was close to BHT and increased with the growth of the content of polysaccharide.The results of thermal stability from thermal analysis（TG）showed that the chemical decomposition of polysaccharide fromLedum was occurred at 190 ℃.The crystallinity of polysaccharide from Ledum was 3.6%,the low degree of crystallinity resulted in a poor molecular rigidity.
出处
《食品与机械》
CSCD
北大核心
2015年第5期206-209,共4页
Food and Machinery
基金
林业公益性行业科研专项(编号:201004081)
关键词
杜香
多糖
抗氧化
物理性质
X射线衍射
热重分析
Ledum palustre L
polysaccharide
antioxidant activity
physical property
XRD
TG
