摘要
目的：探索孟鲁司特钠对生长因子β1（transforming growth factor，TGF-β1）在儿童哮喘中表达的影响及其机制。方法选择哮喘患儿80例，将哮喘患儿按照随机数字表法分成观察组和对照组，每组40例，对照组给予常规治疗，观察组给予孟鲁司特钠治疗。评价两组的临床疗效，测定治疗前后患儿外周血嗜酸性粒细胞计数（EOS）。采用双抗夹心酶联免疫吸附试验（DAS-ELISA）检测治疗前后患儿血清中 TGF-β1、白三烯 E4（LTE4）水平。结果观察组治疗总有效率95．0％，显效28例，有效10例，无效2例；对照组治疗总有效率82．5％，显效23例，有效10例，无效7例，观察组与对照组相比，差异有统计学意义（χ2＝4．200，P ＜0．05）。治疗后两组患儿外周血嗜酸性粒细胞计数均较治疗前显著下降（对照组：χ2＝3．251，P ＜0．05；观察组：χ2＝3.214，P ＜0．05），治疗后观察组与对照组相比，差异有统计学意义（χ2＝3．254，P ＜0．05）；治疗前两组患者血清 TGF-β1、LTE4水平相比，差异无统计学意义（P ＞0．05）。治疗后，与对照组相比，观察组血清 TGF-β1、LTE4水平下降更为显著（TGF-β1：t ＝2．06，P ＜0．05；LTE4：t ＝2．14，P ＜0．05）。结论孟鲁司特钠治疗儿童哮喘有较好的临床效果，可以抑制 TGF-β1在儿童哮喘中的表达，其机制可能与嗜酸性粒细胞、LTE4的水平有关。
Objective To explore the effect and mechanism of montelukast sodium on transforming growth factorβ1 （TGF -β1 ）expression in children with asthma.Methods 80 children with asthma were divided into 2 groups.The control group was treated with common medicine,and the observation group was treated with monte-lukast sodium.The clinical effect of two groups was evaluated.The peripheral eosinophile granulocyte（EOS）count of children was measured before and after treatment.Double antibody sandwich enzyme -linked immunosorbent assay （DAS -ELISA）was used to detect the serum levels of TGF -β1 and leukotriene E4 （LTE4）before and after treatment.Results The total effective rate of montelukast sodium group was 95.0%,remarkable effect in 28 cases, 10 cases were effective,and 2 cases failed.The total effective rate of the control group was 82.5%,23 cases had remarkable effect,effective in 10 cases,ineffective in 7 cases.The effective rate of the montelukast sodium group was statistically higher than the control group（χ2 =4.200,P ＆lt;0.05）.After treatment,the peripheral eosinophil count of patients of two groups significantly decreased than before treatment （control group：χ2 =3.251,P ＆lt;0.05;observation group：χ2 =3.214,P ＆lt;0.05）.There was significant difference between the two groups（χ2 =3.254,P ＆lt;0.05）.Before treatment,serum levels of TGF -1 showed no statistical difference between the two groups （P ＆gt;0.05）.After treat-ment,the levels of serum TGF -1,LTE4 of the observation group decreased more remarkably when compared with the control group （TGF -β1：t =2.06,P ＆lt;0.05;LTE4：t =2.14,P ＆lt;0.05）.Conclusion Montelukast sodium has good clinical effect on childhood asthma.It can inhibit the expression of TGF -1 in childhood asthma,and its mechanism may be related to eosinophils and the level of LTE4.
出处
《中国基层医药》
CAS
2015年第20期3145-3148,共4页
Chinese Journal of Primary Medicine and Pharmacy
