摘要
目的优化维药神香草超临界CO2流体萃取条件,对萃取物的化学成分进行GC-MS分析,为其利用和开发提供科学依据。方法采用正交试验法,以萃取率为指标,考察萃取压力、温度、时间及携带剂用量对萃取效果的影响,确定最优萃取条件,用GC-MS对萃取物进行分析,并通过图谱库检索鉴定化合物结构,用色谱峰面积归一化法确定萃取物中各成分的相对百分含量。结果最佳萃取工艺条件为20 MPa压力,35℃,流量2L/min,萃取1.5h,携带剂与药材用量比为0.75mL∶1g(无水乙醇∶神香草药材=0.75mL∶1g),萃取率达到5.5%,GC-MS分析鉴定出所有34种化合物,其中γ-谷甾醇(31.882%)、二十八烷(19.953%)、亚麻酸(16.279%)、三十六烷(15.939%)、棕榈酸(10.658%)、亚麻酸乙酯(12.471%)、三十一烷(12.215%)、9,12,15-十八烷三烯酸乙酯(9.057%)等化合物含量较高。结论优选的萃取工艺条件稳定、可行,超临界CO2流体萃取物中含较多γ-谷甾醇、亚麻酸、亚麻酸乙酯等目前被认为具有许多生物活性的化合物,具有较大的潜在研究前景。
Objective To optimize the technology parameters of the extraction fromHyssopus officinalis L.（HOL）,a Traditional Uyghur Herbal Medicine,by supercritical CO2 Fluid extraction,and to analyze the extractive components by GC-MS,Thereby providing a scientific basis for the utilization and development.Methods The experiments were designed with the method of orthogonal experiment,and yield of extract was used as value standards.The effect of various extraction pressures,temperature,time and the amount of Carrying agent on yield was investigated to find the optimal conditions.The extractive components were analyzed by GC-MS.The relative content in the extraction was determined by chromatographic peak area normalization.Results The optimized conditions were 20 MPa pressure,35℃,rate of flow 2L/min,extracting duration 1.5h,ethanol∶HOL ratio=0.75mL∶1g,and the extraction rate 5.5%.There were34 components separated and identified from exctracts of HOL by supercritical CO2 Fluid extraction,which mainly includeγ-sitosterol（31.882%）,heneicosanoic（19.953%）,linolenic acid（16.279%）,hexatriacontanoic（15.939%）,palmitic acid（10.658%）,flax ethyl（12.471%）,hentriacontane（12.215%）,9,12,15 octadecane leukotriene ethyl（9.057%）.Conclusion The optimized extraction technology from HOL by supercritical CO2 Fluid extraction was steady and feasible,which had lots of biologically active compounds,ie,γ-sitosterol,linolenic acid,flax and ethyl and etc.with great potential research prospects.
出处
《新疆医科大学学报》
CAS
2015年第11期1372-1375,共4页
Journal of Xinjiang Medical University
基金
新疆少数民族科技人才特殊培养计划科研项目(201323122)
关键词
维药神香草
超临界CO2流体萃取工艺
GC-MS
traditional Uyghur herbal medicine Hyssopus officinalis L.
supercritical CO2 fluid extraction Conditions
GC-MS
