摘要
目的:研究白簕茎多糖(ATMP)对大、小鼠的抗炎镇痛及抗疲劳作用。方法:进行小鼠热板实验,将50只小鼠随机均分为正常对照(等容生理盐水)组、阿司匹林[200 mg/(kg·d)]组和ATMP高、中、低剂量[400、200、100 mg(粗多糖)/(kg·d)]组,测定小鼠痛阈值,考察ATMP的镇痛作用;进行小鼠负重游泳实验,将150只小鼠随机均分为正常对照(等容生理盐水)组、虫草洋参鸡精[以总皂苷计400 mg/(kg·d)]组和ATMP高、中、低剂量[200、100、50 mg(粗多糖)/(kg·d)]组,测定小鼠体质量、力竭游泳时间,并以生化法测定小鼠肝、肌糖原含量及血清中尿素氮(BUN)、乳酸脱氢酶(LDH)、肌酸激酶(CK)含量,考察ATMP的抗疲劳作用;进行角叉菜胶致大鼠足趾肿胀实验,将40只大鼠随机均分为正常对照(等容生理盐水)组、醋酸地塞米松[5 mg/(kg·d)]组、ATMP高、中、低剂量[100、50、25 mg(粗多糖)/(kg·d)]组,记录足趾肿胀度,考察ATMP的抗炎作用。结果:与正常对照组比较,ATMP 400、200、100 mg(粗多糖)/(kg·d)组小鼠痛阈值增高;ATMP 200、100 mg(粗多糖)/(kg·d)组小鼠力竭游泳时间明显延长,肝糖原含量显著增加,血清中CK含量降低;ATMP 200 mg(粗多糖)/(kg·d)组小鼠肌糖原的含量明显增加,血清中BUN和LDH含量减少;ATMP100、50、25 mg(粗多糖)/(kg·d)组大鼠足肿胀度减小,差异均有统计学意义(P<0.01或P<0.05)。但给药前后小鼠体质量并无显著性差异。结论:ATMP对小鼠具有显著的镇痛、抗疲劳作用,对大鼠具有显著的抗炎作用。
OBJECTIVE：To study anti-inflammatory,analgesic and anti-fatigue effects of polysaccharides from Acanthopanan trifoliatus（ATMP） in rats and mice. METHODS：In hot plate experiment,150 mice were randomly divided into normal control group（constant volume of normal saline）,aspirin group [200 mg/（kg·d）],and ATMP high-dose,medium-dose and low-dose groups [400,200,100 mg（gross polysaccharide）/（kg·d）];the threshold of pain was determined,and analgesic effect of ATMP was investigated. 150 mice were included in exhaustive swimming test and then randomly divided into normal control group（constant volume of normal saline）,Chongcao yangshen jijing group [400 mg/（kg·d）by total saponins],and ATMP high-dose,medium-dose and low-dose groups [200,100,50 mg（gross polysaccharide）/（kg·d）];the body weight and exhaustive swimming time of mice were determined,and biochemical process was used to determine the contents of hepatic glycogen and muscle glycogen,and serum levels of BUN,LDH and CK in mice. The anti-fatigue effect of ATMP was investigated. In carrageenan-induced paw swelling experiment,40 rats were divided into normal control group（constant volume of normal saline）,dexamethasone acetate group [5 mg/（kg·d）],ATMP high-dose,medium-dose and low-dose groups [100,50,25 mg（gross polysaccharide）/（kg·d）];the degree of paw swelling was recorded,and anti-inflammatory of ATMP was investigated. RESULTS：Compared with normal control group,the threshold of pain in mice were increased in ATMP 400,200,100 mg（gross polysaccharide）/（kg·d）groups;the exhaustive swimming time of mice were prolonged significantly,and the contents of hepatic glycogen in mice were increased significantly,while serum contents of CK decreased in ATMP 200,100 mg（gross polysaccharide）/（kg·d）groups;the content of muscle glycogen in mice was increased significantly in ATMP 200 mg（gross polysaccharide）/（kg·d）group,while serum contents of BUN and LDH were decreased;the degree of paw swelling in rats was decreased in ATMP 100,50,25 mg（gross polysaccharide）/（kg·d）groups,with statistical significance（P〈0.01 or P〈0.05）. There was no significant difference in body weight of mice before and after medication. CONCLUSIONS：ATMP has significant analgesic and anti-fatigue effects on mice and anti-inflammatory effect on rats.
出处
《中国药房》
CAS
北大核心
2015年第31期4364-4367,共4页
China Pharmacy
基金
广东省建设中医药强省科研课题(No.20151267)
广东药学院药科学院青年教师科研培育基金项目
关键词
白簕茎
多糖
抗炎
镇痛
抗疲劳
大鼠
小鼠
Acanthopanan trifoliatus
Polysaccharide
Anti-inflammatory
Analgesic
Anti-fatigue
Rats
Mice
