摘要
目的:优化昆仑雪菊总皂苷的提取工艺。方法:采用乙醇浸提法;以提取温度、乙醇体积分数、提取时间、料液比为考察因素,以总皂苷提取率为考察指标,在单因素试验基础上设计正交试验进行提取工艺的优化,并进行验证试验。结果:最优提取工艺为昆仑雪菊与60%乙醇液料比为1∶30,在50℃下提取2 h;验证试验表明此工艺下总皂苷平均提取率为6.8%(RSD=0.85%,n=3)。结论:优化工艺可用于昆仑雪菊总皂苷的提取,且工艺稳定。
OBJECTIVE：To optimize the extraction technology of total saponins from Coreopsis tinctoria. METHODS：Ethanol leaching technology was adopted. Based on single factor test,the extraction technology was optimized by orthogonal test using extraction temperature,ethanol volume fraction,extraction time,solid-liquid ratio as factors,extraction rate of total saponins as index. The optimized technology was validated. RESULTS：The optimal technology was that ratio of solid to liquid was 1 ∶ 30（C.tinctoria-60% ethanol）,extracting for 2 h at 50 ℃. Validation test showed that average extraction rate of total saponins was 6.8%（RSD=0.85%,n=3）. CONCLUSIONS：The optimized technology can be used for the extraction of total saponins from C. tinctoria and keep stable.
出处
《中国药房》
CAS
北大核心
2015年第31期4415-4417,共3页
China Pharmacy
关键词
昆仑雪菊
总皂苷
单因素试验
正交试验
提取工艺
Coreopsis tinctoria
Total saponins
Single factor experiment
Orthogonal test
Extraction technology
