摘要
目的观察针刺对抑郁症睡眠障碍(SDD)大鼠海马组织及血清中SOD活性和MDA含量的影响。方法正常组8只SD大鼠常规合笼饲养。32只SD大鼠接受为期21 d的孤养+CUMS法+REM睡眠剥夺法造模,其中28只完成造模,二次分组为模型组、针刺组、药物组、针药组,每组各7只。模型组仅造模不干预;针刺组取印堂、神庭、太冲(双)进行针刺;药物组给予舍曲林和阿普唑仑水溶液灌胃;针药组同时结合针刺组与药物组的两种干预方式;3组均连续干预7 d。结果 SDD造模致大鼠海马组织〔(41.19±5.59)u/m L〕和血清〔(34.57±5.53)u/m L〕的SOD活性较正常组〔(60.19±8.85)、(56.20±13.68)u/m L〕明显下调(P<0.05),且致海马组织〔(5.12±1.22)nmol/mg〕和血清〔(6.22±0.96)nmol/mg〕的MDA含量较正常组〔(3.49±1.02)、(4.35±1.55)nmol/mg〕明显上调(P<0.05);针刺组海马组织〔(52.29±11.37)u/m L〕和血清〔(54.32±6.95)u/m L〕SOD活性均较模型组有明显的上调(P<0.05),且仅针刺组的海马组织〔(3.86±0.80)nmol/mg〕MDA含量较模型组得到了明显的下调(P<0.05);药物组仅上调了血清SOD活性〔(44.70±5.31)u/m L,P<0.05)〕;针药组上调了海马组织〔(51.95±7.58)u/m L〕和血清〔(48.23±6.70)u/m L〕的SOD活性(P<0.05)。结论 SDD造模能明显加速衰老;3种干预手段均有一定的抗衰老作用,其中针刺组最优,针药组次之。
Objective Observation on acupuncture on hippocampus and serum SOD Activity and MDA content of SDD Rats. Methods The 8 normal Sprague Dawley rats were caged together normally. The 32 Sprague Dawley rats were underwent a 21 d solitary raised ＋ CUMS ＋ REM sleep deprivation method modeling. The 28 rats successfully comple-ted the modeling,and divided into model group,acupuncture group,medicine group,acupuncture with medicine group, each group with 7 rats. Model group rats,only modeling without intervention. Acupuncture group rats were acupuncture on acupoint Yintang,Shenting and Taichong（both）each day for 20 min with a 15 s stimulation every 10 min. Medi-cine group rats were gavaged administration with Zoloft and Alprazolam solution. Acupuncture with medicine group combined acupuncture and medicine intervention. Three intervention groups were given intervention for 7 days. Results SOD activity of hippocampal tissue（41. 19 ± 5. 59）u / mL and serum（34. 57 ± 5. 53）/ mL of DD rats was signifi-cantly lower than the normal rats（P 〈 0. 05）,and hippocampus tissue and serum MDA content significantly increased than the normal（60. 19 ± 8. 85,56. 20 ± 13. 68）u / mL rats（P 〈 0. 05）;Acupuncture group’s SOD activity of hipp-ocampal tissue（5. 12 ± 1. 22）nmol/ mg and serum（6. 22 ± 0. 96）nmol/ mg were up regulated than the model group （3. 49 ± 1. 02）,（4. 35 ± 1. 55）nmol/ mg（P 〈 0. 05）,and only acupuncture group’s MDA content in hippocampus has been significantly down regulated（P 〈 0. 05）;Medicine group’s SOD（52. 29 ± 11. 37）u / ml activity of serum （54. 32 ± 6. 95）u / ml were down regulated（P 〈 0. 05）;Acupuncture combined with medicine group’s SOD activity （44. 70 ± 5. 31）u / mL,P 〈 0. 05,of hippocampal tissue（51. 95 ± 7. 58）u / ml and serum（48. 23 ± 6. 70）u / ml were up regulated（P 〈 0. 05）. Conclusion Sleep deprivation depression modeling can significantly accelerate ag-ing;3 ways of interventions on SDD rats have some anti-aging effects. And acupuncture group has the best effect with acupuncture combined with medicine group followed.
出处
《吉林中医药》
2015年第10期1060-1063,共4页
Jilin Journal of Chinese Medicine
基金
国家自然科学基金经费资助(81072878
81303041)
关键词
抑郁症
睡眠障碍
衰老
针刺
靳三针
大鼠
depression
sleep deprivation
aging
acupuncture
Jin’s three technique
rat
