摘要
采用梯度稀释平板分离法从土壤中分离菌种,并通过与研究中心实验室购买和保存的其他11株菌株进行比较,最终筛选出了一株香兰素摩尔转化率和转化效率相对较高的菌株B7,结合菌株形态学特征、生理生化试验结果和菌株16S rRNA序列分析鉴定及系统发育分析结果,最终鉴定该菌株为枯草芽孢杆菌(Bacillus subtilis)。
The bacteria were isolated from soil by gradient dilution plate separation method. In final, a strain B7 with relatively high conversion capability of vanillin was screened by comparing with other 11 strains purchased and preserved in laboratory of research center. Through strain morphological characteristics, physiological and biochemical test results, 16S rRNA sequence identification and phylogenetic analysis results, the strain B7 was inditified as Bacillus subtilis.
出处
《中国酿造》
CAS
北大核心
2015年第10期47-52,共6页
China Brewing
基金
甘肃省食品酶制剂共性关键技术研究创新团队(098TTCA013)
甘肃省技术研究与开发专项计划(1205TCYA034)
甘肃省重点实验室建设计划项目(1309RTSA025)
关键词
阿魏酸
香兰素
筛选
鉴定
ferulic acid
vanillin
screening
identification
