摘要
目的研究转化生长因子β1(TGF-β1)对牙周膜干细胞(PDLSCs)的迁移、粘附和增殖的影响,初步探讨TGF-β1通过影响牙周组织干细胞参与牙周改建的机制。方法分离培养人PDLSCs,鉴定其多向分化潜能。用Transwell法检测TGF-β1对PDLSCs的迁移的影响。用粘附实验检测TGF-β1对PDLSCs粘附能力的影响。用MTT法和生长率法检测TGF-β1对PDLSCs增殖的影响。结果浓度为2.5ng/mL以及10ng/mL的TGF-β1可促进PDLSCs的迁移,而且此作用呈剂量效应关系。粘附实验结果显示,在分别作用2h和4h后,浓度为10ng/mL的TGF-β1可促进PDLSCs的粘附。MTT法的结果证实了TGF-β1可促进PDLSCs的增殖,该作用呈剂量效应关系。再将细胞在含有或者不含有10ng/mLTGF-β1的培养基中培养2、4、6d,对细胞进行计数后发现TGF-β1显著促进PDLSCs的生长。结论 TGF-β1可促进PDLSCs的迁移、粘附和增殖。推测TGF-β1可能通过诱导PDLSCs向牙周组织部位迁移并提高其粘附和增殖能力。
Objective To evaluate the effects of transforming growth factorβ1（TGF-β1）on migration,adhesion and proliferation of periodontal ligament stem cells（PDLSCs）and explore the mechanisms of PDLSCsinduced periodontal remodeling.Methods PDLSCs were isolated and identified from human teeth.The effect of TGF-β1 on migration of PDLSCs was evaluated using transwell migration assay.Cells attachment assay was used to test the effect of TGF-β1 on the adhesion of PDLSCs.In addition,the effect of TGF-β1 on the proliferation of PDLSCs was evaluated by MTT and cell growth rate assay.Results The results showed that TGF-β1 induced the migration of PDLSCs in a dose-dependent manner,improved the adhesion and proliferation of PDLSCs.So we propose that TGF-β1 may promote periodium remodeling by inducing PDLSCs migration,following adhesion and proliferation in these areas.Conclusion This study demonstrated for the first time that TGF-β1 increases the adhesion and migration of PDLSCs in vitro.The signal pathway is involved in the TGF-β1-induced migration of PDLSCs and the mechanical-chemical interaction during the orthodontic periodontal remodeling will be researched in our further studies.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2015年第6期782-786,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.81200822)
中央高校基本科研业务费专项资金资助项目(No.2012jdhz27)
陕西省自然科学基金资助项目(No.2012JQ4004)~~
关键词
牙周膜干细胞
TGF-β1
迁移
粘附
增殖
periodontal ligament stem cell
TGF-β1
migration
adhesion
proliferation
