摘要
目的观察黑果枸杞对D-半乳糖诱导的小鼠衰老模型的学习记忆力及抗氧化能力的影响。方法注射5%D-半乳糖制备小鼠衰老模型,灌胃给予黑果枸杞6周后,水迷宫法测定模型小鼠的空间学习记忆能力,试剂盒法测定脑中丙二醛(MDA)、过氧化脂质(LPO)含量及超氧化物歧化酶(SOD)活力,以及血清中谷胱甘肽过氧化物酶(GSH-Px)、MDA含量及SOD活力。结果黑果枸杞能缩短衰老模型小鼠在水迷宫的逃避潜伏期、游泳总路程、近台区停留时间、远台区停留时间、远台区游泳路程,增加衰老小鼠平均游泳速度及近台区的游泳路程;降低模型小鼠血清中MDA含量,升高血清中GSH-Px含量和SOD活力;降低模型小鼠脑中MDA、LPO含量,升高脑中SOD活力。结论黑果枸杞能改善衰老小鼠学习记忆能力,提高机体的抗氧化能力,具有一定的延缓衰老的作用。
Objective To study the effect of Lycium ruthenicum Murr. on the learning,memory and antioxi-dant ability of aging mice induced by D-galactose. Methods Aging model was induced by subcutaneously injecting mice with D-galactose. At 6 weeks after administration of aqueous extract of Lycium ruthenicum Murr.,the water maze was used to determine the function of learning and memory,the analytic kit was used to measure the activity of super-oxide dismutase （ SOD）,the contents of malondialdehyde （ MDA） and lipid peroxidation （ LPO） in brain of mice,as well as the activity of SOD,the contents of MDA and glutathione peroxidase （ GSH-Px） in serum of mice. Results Lycium ruthenicum Murr. could reduce the escape latency,swimming total path,shorten the time in the near station,sig-nificantly shorten the time and swimming path in the distant station area to stay,increase the residence swimming path in the area close to the table and the average swimming speed of aging model mice in a water maze. Besides,it could reduce MDA content and increase GSH-Px content and SOD activity in serum,increase SOD activity and reduce LPO and MDA contents in brain （P〈0. 05,P〈0. 01）. Conclusion Lycium ruthenicum Murr. has anti-aging effect on ag-ing model mice,and the mechanism may be related to improving learning and memory and enhancing antioxidant ability in aging mice.
出处
《实用药物与临床》
CAS
2015年第10期1147-1150,共4页
Practical Pharmacy and Clinical Remedies
基金
国家自然科学基金青年基金(81102885)
关键词
黑果枸杞
抗衰老
抗氧化
D-半乳糖
Lycium ruthenicum Murr.
Anti-aging
Antioxidant
D-galactose
