摘要
CH5026是携带中间偃麦草抗病基因的渗入系。为了更好地利用CH5026,拓宽小麦抗性育种资源,对其抗条锈性来源和遗传模式进行了分析,对抗性基因进行了染色体定位并构建了遗传连锁图谱。在苗期和成株期对CH5026及其亲本分别接种条锈菌流行小种CYR31、CYR32和CYR33。结果表明,CH5026在苗期和成株期对这3个条锈菌小种均表现出免疫或近免疫,且与其抗性供体TAI7045及其野生亲本中间偃麦草抗病侵染型相似。对其与感病品种(系)的杂交后代F1、F2、F2:3和BC1群体接种CYR32进行成株期抗性遗传机制分析,证实CH5026对CYR32的抗性由1对显性核基因控制。基因组原位杂交未检测到外源DNA杂交信号。用569对SSR引物对CH5026/台长29的192个F2群体进行分析,发现3个与抗性基因连锁的SSR标记:Xgwm210、Xwmc382和Xgpw7101,抗性基因位点与两翼邻近连锁标记Xwmc382和Xgpw7101的遗传距离分别为6.0,4.7 c M。利用中国春缺四体、双端体材料将该基因及其连锁标记定位在染色体2AS上。通过基因来源及连锁分子标记多态性比较,这个抗条锈病基因与已知定位于染色体2AS上的抗性基因不同,很可能是一个新的抗条锈病新基因,暂将其命名为Yr CH5026。
CH5026 is a stable introgression line carrying resistance genes of Thinopyrum intermedium. To better utilize CH5026,increase the genetic resources,this study were to characterize the origin of the resistance,determine the inheritance of resistance,locate the chromosome and construct the linkage map. Using three prevalent races of stripe rust CYR31,CYR32 and CYR33,Th. intermedium（ accession Z1141）,CH5026 and its resistant parent amphiploid TAI7045 as well as all wheat parents were evaluated by inoculated races in the seedling stage and adult stages. The result showed that CH5026 and its donor TAI7045 as well as the wild parent were resistant at the seedling and adult stages to CYR31,CYR32 and CYR33,whereas all the wheat parents involved were susceptible,indicating that the CH5026 was possibly derived from Th. intermedium. No Th. intermedium chromatin has been detected according to genome in situ hybridization assay. Genetic analysis on the F1,F2,F2∶ 3and BC1 populations from stripe rust resistant line CH5026 revealed that a single dominant gene resistance to CYR32. A total of 192 F2 populations from the cross CH5026 / Taichang 29（ TC 29） was screened with 569 wheat SSR primer pairs to detect molecular markers linked to resistance gene. Three SSR primers,Xgwm210,Xwmc382 and Xgpw7101 were identified to be linked to the resistance gene. The two flanking markers were Xwmc382 and Xgpw7101,genetic distances were6. 0,4. 7 cM,respectively. Using the Chinese Spring（ CS） nulli-tetrasomic and ditelosomic lines,the resistance geneand its linked markers were assigned to chromosome arm 2AS. Based on the source of the Yr genes and ploymorphism of linked molecular markers,the stripe rust resistance gene in CH5026 was likely different from the known resistance gene on chromosome 2AS. The gene might be a novel stripe rust resistance gene,this new resistance gene is temporarily named Yr CH5026.
出处
《华北农学报》
CSCD
北大核心
2015年第5期7-15,共9页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31171839)
山西省国际科技合作计划项目(2013081007)
山西省科技攻关项目(20130311001-5)
山西省科技创新团队建设项目(2013131025)
关键词
小麦
中间偃麦草
渗入系
条锈病
分子定位
Wheat
Thinopyrum intermedium
Introgression line
Stripe rust
Molecular mapping
