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时间分辨荧光免疫技术在梅毒特异性抗体筛查中的应用研究 被引量:2

Application analysis of time-resolved fluorescence immunoassay technology in syphilis specific antibody screening
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摘要 目的探讨时间分辨荧光免疫技术(TRFIA)在梅毒特异性抗体检测中的应用价值。方法收集240例梅毒患者和150例健康者血清标本,采用TRFIA、梅毒螺旋体酶联免疫吸附试验(TP-ELISA)、梅毒螺旋体明胶颗粒凝集试验(TPPA)进行梅毒特异性抗体检测,比较检测灵敏度、特异度及阳性率。结果TRFIA、TP-ELISA、TPPA检测灵敏度分别为100.00%、98.75%、97.92%,比较差异无统计学意义(P>0.05);特异度分别为99.33%、98.67%、100.00%。TRFIA检测假阳性率为0.67%,假阴性率为0.00%;TP-ELISA检测假阳性率为1.33%,假阴性率为1.25%,TRFIA检测假阳性率和假阴性率均低于TP-ELISA(P<0.05)。结论 TRFIA检测梅毒特异性抗体灵敏度和特异度高,结果准确可靠,可用于梅毒特异性抗体的常规筛查,值得推广应用。 Objective To investigate the application of time-resolved fluorescence immunoassay(TRFIA)in the detection of specific antibody of syphilis.Methods Specific antibody of syphilis was detected in serum samples of 240 cases of syphilis and 150 healthy subjects by TRFIA,Treponemal pallidum particle agglutination(TPPA)and Treponemal pallidum enzyme linked immunosorbent assay(TP-ELISA).The sensitivity,specificity and positivity of these three methods were compared.Results The sensitivity of TRFIA,TP-ELISA,TPPA were 100.00%,98.75% and 97.92%,without significantly differences(P〈0.05),and the specificity were 99.33%,98.67%and 100.00%.The false positive rate of TRFIA was 0.67%,and the false negative rate was 0.00%.The false positive rate of TP-ELISA was 1.33%,and the false negative rate was 1.25%.False positive rate and false negative rate of TRFIA were lower than TP-ELISA(P〈0.05).Conclusion TRFIA could be with high sensitivity and specificity in syphilis specific antibody test,and could be used for routine screening of syphilis specific antibody.
出处 《国际检验医学杂志》 CAS 2015年第21期3117-3118,共2页 International Journal of Laboratory Medicine
关键词 梅毒特异性抗体 时间分辨荧光免疫技术 酶联免疫吸附试验 明胶颗粒凝集试验 对比分析 syphilis specific antibody TRFIA TP-ELISA TPPA comparative analysis
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