摘要
目的为益肾胶囊建立定性定量方法。方法采用薄层色谱法鉴别益肾胶囊中丹参、姜黄,运用高效液相色谱法测定马钱苷、苦杏仁苷、紫丁香苷的含量。结果丹参、姜黄的薄层色谱斑点清晰、无拖尾现象,马钱苷、苦杏仁苷、紫丁香苷依次在4.05~101.3μg·m L-1（r=0.9997）、7.71~192.8μg·m L-1（r=0.9995）、7.65~191.3μg·m L-1（r=0.9997）与峰面积均呈良好的线性关系,平均回收率依次为99.7%、99.6%、98.9%,RSD依次为0.5%、0.6%、1.1%。结论薄层鉴别法和含量测定方法操作简单、专属性强、无阴性干扰、重复性好,可以很好地控制益肾胶囊的质量。
Objective To establish a qualitative and quantitative method for kidney capsules. Methods Salvia miltior- rhiza radix and Curcuma longa rhizome of kidney capsules were identificated by TLC. HPLC was used to determine the content of loganin, laetrile and syringin. Results TLC chromatography spots of Salvia miltiorrhiza radix and Cur- cuma longa rhizome were clear, without tailing phenomena. The linear ranges of loganin, laetrile and syringin were 4.05 - 101.3 μg· mL^-1 （r = 0.9997）, 7.71-- 192.8μg·mL^-1 （r= 0.9995）, and 7.65 - 191.3 μg· mL^- 1 （r = 0.9997）; the average recovery rates were 99.7%, 99.6% and 98.9%, with RSD of 0.5%, 0.6% and 1.1%, respectively. Conclusion TLC method and HPLC method are simple, specific, and reproducible, without no negative interference, which can better control the quality of kidney capsules.
出处
《中南药学》
CAS
2015年第10期1089-1092,共4页
Central South Pharmacy
基金
中药现代化攻关计划项目(编号:2012K19-03-07)
