摘要
目的 探究基于Th1/Th2细胞平衡前泌通片对大鼠慢性非细菌性前列腺炎的治疗作用机理.方法60只SD雄性大鼠随机分为空白组、模型组、阳性对照组、前泌通片低中高剂量组,每组10只.除空白组,余各组建立慢性非细菌性前列腺炎模型.建模成功后,阳性对照组给予前列通瘀胶囊混悬液灌胃,前泌通片低中高剂量组分别给予不同剂量前泌通混悬液灌胃,空白组给予生理盐水灌胃,连续灌胃4周,末次给药后24h,股动脉放血处死大鼠,光镜下观察前列腺组织病理改变,免疫组化法检测IFN-γ和IL-4并计算IFN-y/IL-4比值.结果 与模型组比,前泌通片高中剂量组组织炎症明显减轻,前泌通片高剂量组IFN-γ和IL-4含量显著降低（JP＜0.01）,前泌通片中低剂量组IL-4含量显著降低（P＜0.01）,前泌通片高中低剂量组IFN-y/IL-4比值显著升高（P＜0.01）.结论 前泌通片可减轻慢性非细菌性前列腺炎大鼠组织炎症和下调IFN-γ、IL-4含量,上调IFN-y/IL-4比值.
Objective To explore therapeutic effects of Qian Mi Tong on chronic nonbacterial prostatitis based on the balance of Thl/Th2 cells. Mothods A total of 60 SD rats were randomly divided into six groups such as the control group, the model group, positive group and Qian Mi Tong high/medium/low dose group, 10 rats each group. The rat models with chronic nonbacterial prostatitis were established in all groups except the blank group. The rat models in the positive group were intragastdcally administrated with Qinn Lie Tong Yu capsule. The rat models in qian Mi Tong high/medium/low dose group were intragastrically administrated with qian Mi Tong tablet, and the rats in the control group with normal saline. The forced feeding lasted for 4 weeks. The rats were killed by femoral artery cutting twenty four hours after last time forcd feeding. Pathological changes in prostatic tissue were observed under the light microscope and the expressions of the IFN- y and IL-4 were measured by immunohistochemistry. Roaults Compared with that of the model group, tissue inflammationwas significantly reduced in Qian 114i Tong high/medium dose group. There is an obvious decrease in expressions of IFN- y and IL-4 in Qian Mi Tong high dose group （P〈0.01） and also decrease in IL-4 content in Qian Mi Tong medium/low dose group （P〈0.01）. The ratio of IFN- y/IL-4 was significantly increased in Qian Mi Tong high/medium/low dose group （P〈0.01）. Conohmion Qian Mi Tong can relieve the tissue inflammation of rats with chronic nonbacterial prostatitis, downreffulate the IFN- 3＇ and IL-4 expression, and increas the ratio ofIFN- T/IL-4.
出处
《中国男科学杂志》
CAS
CSCD
2015年第8期13-17,共5页
Chinese Journal of Andrology
基金
成都中医药大学附属医院科技基金项目(编号:2011-D-YY-22)
