摘要
目的 评价全氟化碳不同给药时机对兔急性肺损伤（ALI）的影响.方法 新西兰大白兔18只,体重1.8～ 2.4 kg,50～ 60日龄,雌雄不拘.采用随机数字表法,将其分为3组（n=6）：ALI组、全氟化碳预防性给药组（PP组）和全氟化碳治疗性给药组（PA组）.ALI组机械通气60 min时,于5 min内颈内静脉注射油酸0.1 ml/kg制备ALI模型,分3次注射完毕;PP组吸人全氟化碳2ml· kg-1·h-160 min,然后制备ALI模型;PA组机械通气60 min时制备ALI模型,模型制备成功后吸入全氟化碳2 ml· kg-1·h-160 min.分别于麻醉稳定30 min（T0）、机械通气60 min或全氟化碳预防性给药后60 min（T1）、造模后30 min（T2）、造模后60 min（T3）、造模后90 min（T4）和造模后150 min（T5）时,取动脉血样行血气分析,计算氧合指数.于T5时,收集支气管肺泡灌洗液（BALF）,然后处死大鼠,取肺组织,采用ELISA法检测BALF中TNF-α和IL-1β的浓度,计算肺组织湿重/干重比值（W/D比值）,采用Western blot法检测Toll样受体4（TLR4）和NF-κB p65的表达水平.结果 与T0和T1时比较,3组T2-5时氧合指数均降低（P＜0.05）;与ALI组比较,PP组T2-5时氧合指数升高,BALF中TNF-α和IL-1β的浓度降低,肺组织W/D比值降低,TLR4和NF-κB p65的表达下调,PA组T5时氧合指数升高（P＜0.05）,其余指标差异无统计学意义（P＞0.05）.结论 预防性吸人全氟化碳2ml· kg-1·h-160 min可减轻兔ALI.
Objective To evaluate the effects of perfluorocarbon administered at different time points on acute lung injury （ALI） in rabbits.Methods Eighteen New Zealand white rabbits, weighing 1.8-2.4 kg, aged 50-60 days, were randomly divided into 3 groups （n=6 each） using a random number table： ALI group, preventive administration of perfluorocarbon group （group PP） , and therapeutic application of perfluorocarbon group （group PA）.In group ALI, after the rabbits were mechanically ventilated for 60 min, ALI was induced with oleic acid 0.1 ml/kg injected via the internal jugular vein in three times within 5 min.In group PP, perfluorocarbon was inhaled at 2 ml · kg-1 · h-1 for 60 min, and then ALI was induced.In group PA, after the rabbits were mechanically ventilated for 60 min, ALI was induced, and then perfluorocarbon was inhaled at 2 ml · kg-1 · h 1 for 60 min.After 30 min stabilization （baseline, T0） , at 60 min of ventilation or at 60 min after preventive administration of perfluorocarbon （T1） , and at 30, 60, 90 and 150 min after ALI（T2-5）, arterial blood samples were collected for blood gas analysis.Oxygenation index was calculated.Broncho-alveolar lavage fluid （BALF） was collected to determine the concentrations of tumor necrosis factor-alpha （TNF-α） and interleukin-1 beta （IL-1β） （by ELISA） at T5.The animals were then sacrificed, and lungs were removed for determination of the expression of Toll-like receptor 4 （TLR4） and nuclear factor kappa B p65 （NF-κB p65） （by Western blot）.Wet to dry lung weight ratio （W/D ratio） was calculated.Results Oxygenation index was significantly decreased at T2-5 than at T0 and T1 in the three groups.Compared to group ALI, oxygenation index was significantly increased at T2-5, the concentrations of TNF-α and IL-1β in BALF were decreased, W/D ratio was decreased, and the expression of TLR4 and NF-κB p65 was down-regulated in group PP, and oxygenation index was significantly increased at T5, and no significant change was found in the other parameters in group PA.Conclusion Preventive inhalation of perfluorocarbon 2 ml · kg-1 · h-1 can mitigate ALI in rabbits.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2015年第8期1011-1013,共3页
Chinese Journal of Anesthesiology
基金
广东社会发展领域科技计划（20118031800192）
广州市科技和信息化局科学研究专项（2013J4100098）
关键词
氟碳化合物
呼吸窘迫综合征
成人
Fluorocarbons
Respiratory distress syndrome,adult
