期刊文献+

HGF/c-met传导通路在子宫内膜癌发生发展中的作用及意义 被引量:3

Effect and significance of HGF/c-met pathway in occurrence and development of endometrial cancer
下载PDF
导出
摘要 目的检测HGF/c-met传导通路对Survivin、XIAP表达的影响,探讨HGF/c-met通路在子宫内膜癌发生发展中的作用机制。方法培养ER表达水平不同的子宫内膜癌细胞系HEC-1B(ER低表达)和Ishikawa(ER高表达),通过MTT法检测不同浓度HGF对两种细胞增殖率的影响;AnnexinV-FITC法检测不同浓度HGF对细胞凋亡率的影响。Westem Blot检测Survivin及XIAP蛋白的表达水平。结果 HGF可促进子宫内膜癌细胞的增殖,在80 n咖L内具有剂量依赖性,在72 h内具有时间依赖性(P<0.05);当HIGF浓度为40 ng/mI时,HEC-1B细胞24 h增值率达到(87.0±0.02)%,为最适诱导浓度。Survivin、XIAP mRNA及蛋白的表达随着HGF浓度的增高而上调,上调作用具有剂量效应关系。HGF在ER低表达的HEC-1B细胞中作用明显高于Ishikawa细胞,差异具有统计学意义(P<0.05)。结论 HGF/c-met传导通路的激活可促进子宫内膜细胞增殖,抑制细胞凋亡,HGF/c-met可能是调控子宫内膜癌发生发展的分子靶点。 Objective To detect the influence of HGF/c-met pathway on expression of survivin and XIAP and to discuss the action mechanism of HGF/c-met pathway in occurrence and ddevelopment of endometrial cancer.Methods Cell lines of endometrial cancer with different ER expression level were cultivated:HEC-1B(ER low expression) and Ishikawa(ER high expression).The effects of HGF in various concentrations on cell proliferation rate of both cell lines were detected by MIT method,and effects on cell apoptosis rate were detected by AnnexinV-FITC method.The expression levels of survivin and XIAP were measured by western blot.Results HGF facilitated the proliferation of endometrial cells,which was dose-dependent within 80 ng/mL,and time-dependent within 72 h(P〈0.05):When HCF=40ng/mL,the 24 h proliferation rate of HEC-1B cells reached(87.0±0.02)%,and 40 ng/mL was the optimum inducing concentration.The mRNA and protein expressions of surviving and XIAP were up regulated by the rising HGF concentration,which had a dose-effect relationship.The effect of HGF on ER low expression HEC-1B cells was evidently higher than that on Ishikawa cells,the difference was statistically significant(P〈0.05).Conclusion The activation of HGF/c-met pathway can facilitate the proliferation and inhibit the apoptosis of endometrial cells.HGF/c-met is probably the molecular target regulating the occurrence and development of endometrial cancer.
出处 《中国现代医生》 2015年第32期1-7,共7页 China Modern Doctor
基金 山西省回国留学人员科研资助项目(2014-082)
关键词 子宫内膜癌 HGF/c-met传导通路 SURVIVIN XIAP Endometrial cancer HGF/c-met pathway Survivin XIAP
  • 相关文献

参考文献20

  • 1JonathanS,BerekN.妇科学[M].北京:人民卫生出版社,2008.
  • 2Mendes KN, Nicorici D, Cogdell D, et al. Analysis of sig- naling pathways in 90 cancer cell lines by protein lysate array[J]. Journal of Proteome Research,2007,6(7): 2753- 2767.
  • 3Su HY, Lai HC,Lin YW,et al. Epigenetic silencing of SFRP5 is related to malignant phenotype and chemoresis- tance of ovarian cancer through Wnt signaling pathway[J]. International Journal of Cancer, 2010,127 (3) : 555-567.
  • 4Maulik G,Shrikhande A,Kijima T,et al. Role of the hep- atocyte growth factor receptor, e-met,in oncogenesis and potential for therapeutic inhibition[J]. Cytokine & Growth Factor Reviews, 2002,13 ( 1 ) : 41-59.
  • 5Kammula US, Kuntz E J, Francone TD, et al. Molecular co- expression of the c-met oncogene and hepatocyte growth factor in primary colon cancer predicts tumor stage and clinical outcome [J]. Cancer Letters, 2007,248 (2) : 219- 228.
  • 6Hunter AM,LaCasse EC,Korneluk RG. The inhibitors of apoptosis (IAPs) as cancer targets [J]. Apoptosis, 2007,12 (9) : 1543-1568.
  • 7Takeuchi H, Kim J, Fujimoto A, et al. X-linked inhibitor of apoptosis protein expression level in colorectal cancer is regulated by hepatocyte growth Factor/C-met pathway via Akt signaling[J]. Clinical Cancer Research,2005,11 (21) : 7621-7628.
  • 8Guseva NV, Dessus-Babus SC, Whittimore JD, et al. Char- acterization of estrogen-responsive epithelial cell lines and their infectivity by genital Chlamydia traehomatis[J]. Microbes and Infection, 2005,7 (15) : 1469-1481.
  • 9Ma PC, Maulik G, Christensen J, et al. c-met: Structure, functions and potential for therapeutic inhibition[J]. Can- cer and Metastasis Reviews, 2003,22(4) : 309-325.
  • 10Abidoye Oyewale, Murukurthy Nadh, Salgia Ravi. Review of clinic trials:Agents targeting c-Met[J]. Rev Recent Clin Trials, 2007,2 (2) : 143 - 147.

二级参考文献14

  • 1徐艳红,温宏武.雌激素受体亚型ERα和ERβ在子宫内膜癌中表达的研究[J].中国医刊,2006,41(8):31-33. 被引量:10
  • 2Jemal A, Siegel R, Xu J, et al. Cancer Statistics 2010. CA Cancer J Clin, 2010, 60: 277- 300.
  • 3Ben Arie A, Lavie O, Gdalevich M, et al. Temporal pattern of recurrence of stage I endometrial cancer in relation to his- tological risk factors. Eur J Surg Oncol, 2012, 38: 166 - 169.
  • 4Shih HC, Shiozawa T, Kato K, et al. Immunohistochemical expression of cyclins, cyclin - dependent kinases, tumor - suppressor gene products, Ki - 67, and sex steroid receptors in endometrial carcinoma: positive staining for cyclin A as a poor prognostic indicator. Hum Pathol, 2003, 34: 471-478.
  • 5Jeon YT, Park IA, Kim YB, et al. Steroid receptor ex pressions in endometrial cancer: clinical significance and epidemio- logical implication. Cancer Lett, 2006, 239: 198- 204.
  • 6Erkanli S, Kayaselcuk F, Kuscu E, et ak Expression of sur- vivin, PTEN and p27 in normal, hyperplastic, and cardnom- atous endometriurr: Int J Gynecol Cancer, 2006, 16: 1412- 1418.
  • 7Graesslin O, Bastaraud SC, Lorenzato M, et al. Fluorescen- cein situ hybridization and immunohistochemical analysis of p53 expression in endometrial cancer: prognostic value and- relation to ploidy. Ann Surg Oncol, 2008, 15: 484- 492.
  • 8Keam B, Im SA, Lee KH, et al. Ki- 67 can be used for fur ther classification of triple negative breast cancer into two subtypes with different response and prognosis. Breast Cane- erRes, 2011, 13: R22.
  • 9Chen YL, Huang CY, Chien TY, et al. Value of pre-opera tive serum CA125 level for prediction of prognosis in patients with endometrial cancer. Aust N Z J Obstet Gynaecol, 2011, 51: 397-402.
  • 10Wang XF, Wu YH, Wang MS, et al. CEA, AFP, CA125, CA153 and CA199 in malignant pleural effusions predict the cause. Asian Pae J Cancer Prev, 2014, 15: 363- 368.

共引文献17

同被引文献28

引证文献3

二级引证文献18

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部