摘要
目的：制定灵丹多糖胶囊中多糖的含量测定方法。方法：采用苯酚-硫酸显色法在490 nm波长处测定灵丹多糖胶囊中多糖的含量。结果：以葡萄糖作为对照品其浓度在0.12~0.20 mg/m L之间与吸光度有良好的线性关系,A=3.9635 C＋0.0013,r=0.9999,精密度考察RSD为0.10%,稳定性考察RSD为0.12%,稳定性考察RSD为0.12%,平均加样回收率为99.95%,RSD为0.23%,三批样品多糖平均含量为5.22%。结论：苯酚-硫酸显色法操作简便、准确,可作为该胶囊制剂中多糖含量的控制方法。
The content of Polysaccharide in Lingdan Polysaccharide Capsules was determined. Method： Uses phenol sulfuric acid method to determine in Lingdan Polysaccharide Capsules in the 490 nm wave strong point the Polysaccharide content.Result： With glucose as the reference substance content in 0. 12 ~ 0. 20 mg / m L have good linear relationships between,A= 3. 9635 C ＋ 0. 0013,r = 0. 9999. The precision of the RSD wss 0. 10 %,The stability of RSD wss 0. 12 %,The stability of RSD wss 0. 12 %,the average recovery was 99. 95 %,the RSD was 0. 23 %. The average content of polysaccharide in the three batches was 5. 22 %. Conclusion： Phenol-sulfuric acid method wss simple and accurate,and can be used as a method to control the content of polysaccharides in the capsule.
出处
《山东化工》
CAS
2015年第20期63-64,68,共3页
Shandong Chemical Industry
关键词
灵丹多糖胶囊
多糖
苯酚-硫酸法
含量测定
lingdanduotang capsules
polysaccharide
phenol sulfuric acid method
determination
