摘要
本研究通过比色条件NaNO2,Al(NO3)3和NaOH的加入量和静置时间单因子筛选、多因子正交试验和方法学考察,建立了NaNO2-Al(NO3)3-NaOH体系分光光度法测定沙棘叶总黄酮含量的优化方法.结果得出测定沙棘叶总黄酮含量的步骤是:取1mL样液于烧杯中,加70%的乙醇1mL,再加入5%的亚硝酸钠溶液0.5mL,摇匀,静置6min;再加入10%的硝酸铝溶液0.5 mL,摇匀,静置6 min;然后加入4%的氢氧化钠溶液6 mL,摇匀,放置15min后,以70%乙醇作参比溶液,于波长510nm处测定吸光值.测得沙棘总黄酮含量为2.049%,比优化前提高1.337倍,均达到《中国药典》规定的标准.该法稳定性和重现性好,精密度和回收率高,并且设备简单、操作便捷,可为其他材料总黄酮的测定提供参考.
A method for determination of total flavonoids in Hippophe rhamnoids L leaves is established,by singlefactor screening, multiple-factor orthogonal test and methodology examination of NaNO2-Al（NO3）3-NaOH system spectrophotometry.The single factor includs reaction volume and reaction time.The results show that the optimization methods are：Take 1mL sample solution in the beaker,add70% ethanol 1mL and 5% NaNO20.5mL,shake well,react 6min.Then add 10% Al（NO3）30.5mL,shake well,react 6 min;After that add 4% NaOH 6 mL,shake well,react 15 min,determine absorbance at 510 nm with 70% ethanol as reference solution.The methodology shows that the method has good stability and reproducibility,high precision and recovery.It is more suitable for the determination content of total flavonoids in Hippophe rhamnoids L leaves,which has simple equipment,convenient operation and high accurate.using this method,the content of total flavonoids is 2.049%,which is 1.337 times than before optimized.Both of then reach to the regulation standard of ＂China pharmacopoeia＂.This method can be looked as a reference method for the determination of total flavonoids in other materials.
出处
《西北师范大学学报（自然科学版）》
CAS
北大核心
2015年第6期94-97,104,共5页
Journal of Northwest Normal University(Natural Science)
基金
内蒙古自治区教育厅高等学校科学技术研究项目(NJZC14407)
关键词
沙棘叶
总黄酮
含量测定
分光光度法
正交试验
Hippopherhamnoids L leaves
total flavonoids
content determination
spectrophotometry
orthogonal test
