摘要
【目的】优选补肾益智方有效部位的提取工艺。【方法】以有效部位得率、二苯乙烯苷和蛇床子素含量为工艺优选指标,采用层次分析法分析各指标权重;以加水量、提取时间、提取次数为考察因素,针对优选指标的综合评分,通过正交试验优选补肾益智方有效部位的提取工艺。【结果】优选的补肾益智方提取工艺为:加药材8倍量的纯水,煎煮3次,每次1.5 h。经验证,优选工艺的综合评分高于正交设计试验结果。【结论】优选的提取工艺可行,可为补肾益智方的制备工艺提供实验依据。
Objective To optimize the extraction process for the effective fraction of Bushen Yizhi（ BSYZ）formula. Methods Yield of the effective fraction and contents of two marker compounds 2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside and osthole assayed by high performance liquid chromatography（HPLC） were used as the optimizing indexes, and weighted coefficient of the indexes was analyzed by analytic hierarchy process（AHP）. Ratio of liquid-to-solid, extraction time, and extraction times were used to screen the optimal conditions by orthogonal test. Results The optimized extraction process of the effective fraction of BSYZ formula was as follows： extracting with 8-fold water for three times, and boiling for 1.5 hours each time.The verification test showed that the comprehensive scores of the optimized extractive process were higher than those obtained in the orthogonal test. Conclusion The optimized extraction process and conditions are practical in the manufacture of the effective fraction of BSYZ formula.
出处
《广州中医药大学学报》
CAS
2015年第6期1102-1106,1110,共6页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
"重大新药创制"广东省重大科技专项(编号:2012A080202017)
广东省华南中医药协同创新中心创新中药研发团队项目(编号:E1-KFD015141K05)
关键词
补肾益智方
有效部位
层次分析法
正交试验
色谱法
高压液相
Bushen Yizhi formula
Effective fraction
Analytic hierarchy process
Orthogonal test
Chromatography
high performance liquid
