摘要
目的 建立吲哚美辛的紫外分光光度法,并用于吲哚美辛片中吲哚美辛含量及肠溶片释放度的测定.方法 采用紫外分光光度法,以磷酸盐缓冲液（pH7.2）-甲醇（1：1）为溶剂,最大吸收波长320 nm.结果 吲哚美辛标准品溶液在10 -35 μg/ml的浓度范围内,A=0.0195C＋0.0554（μg/ml）线性关系良好,相关系数为0.9995（n=6）;样品在7h内稳定,RSD％为0.25％;平均回收率分别为95.96％,96.85％,98.82％,RSD分别为0.31％,0.36％,0.15％.结论 该含量测定方法准确,快速,简单,可靠,可用于吲哚美辛片含量及肠溶片释放度的测定,较适用于生产过程中的质量控制.
Objective ： To establish a Uv - vis method for Indom - metacin, and apply it into the determin of release ratelof Indom metacin Enteric - coated Tablets. Method： Adopting Uv - vis phosphate buffer solution （Ph7.2） - alcohl（ 1：1 ）as dissolvant to determin its absorbance at the biggest assimilate wavelength. Resuhs：Indom metacin＇s standard substancedissolvent in the 10 - 35 ixg/ml concentration renge , A = 0. 0195 C ＋ 0. 0554 （i.tg/ml） （ r = 0. 9995 ） good liner ralation. Thesample will be stabilization in 7h （ RSD = 0.25% ）, the average of Indom metacin Enteric - coated Tablets （ rt = 6 ） were95.96% （RSD = 0.31% ） ,98.85% （ RSD = 0. 36% ） ,98.82% （ RSD = 0. 15% ）. Conclusion： The method is accurate ,convienent, sensitive and reliable with high reproducibility and used for content determination of release ratel of Indommetacin Enteric -coated Tablets and management control in the productive process.
出处
《泰山医学院学报》
CAS
2015年第8期884-887,共4页
Journal of Taishan Medical College
