摘要
为深入研究小麦U-BOX基因的功能,以周麦22和周麦24为材料,利用RT-PCR技术克隆小麦的Ta-UBX1基因,对其序列结构、基因表达和进化特性进行分析。结果表明,小麦Ta-UBX1基因全长2 059 bp,编码553个氨基酸,在其序列的N端和C端分别具有UBA_like保守域和UBX结构域,属于泛素途径中的典型U-BOX蛋白,并且与其他禾本科植物同源的U-BOX蛋白具有高度的序列相似性;该基因在小麦叶片、小花、幼穗、根、种子中均有表达,但以小花和幼穗中的表达量较高,叶片中表达量较低;在花药发育的四分体期和单核早期表达量最高,单核后期到三核期表达量逐步降低,推测该基因表达可能与小麦花发育,尤其花药发育密切相关。本研究可为探究该基因的功能奠定分子基础。
In order to study the function of the wheat U-BOX gene,the full-length c DNA of a novel UBX domaincontaining gene,designated as Ta-UBX1,was obtained from wheat varieties Zhoumai 22 and Zhoumai 24 using reverse transcription polymerase chain reaction（ RT-PCR） technology. The sequence and expression patterns as well as evolution characteristics of Ta-UBX1 were analyzed. The results showed Ta-UBX1 was 2 058 bp in length,encoded a553 amino acid protein that contained typical UBA_ like and UBX domain in its ＂N-terminal ＂ and ＂C-terminal ＂,respectively,which belonged to UBX protein family in ubiquitin regulation pathway,and the amino acid sequence of TaUBX1 protein has higher similarity to the known UBX domain-containing proteins from other gramineous plants,showed high conservative in gramineous plants. Ta-UBX1 gene exhibited significant expression variations in different tissues.Which was more abundant in spikelet flowers and young ear than in other tissues,and showed higher expression amount from early tetrad stage to mononuclear stage,then gradually reduced from late-mononuclear to trinucleate stage during anthers development,which suggested it might be involed in floral development,especially in male gametophyte development in wheat（ Triticum aestivum L.）. These results provide a molecular basis to understand the function of TaUBX1 gene.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第10期1858-1866,共9页
Journal of Nuclear Agricultural Sciences
基金
国家863计划项目(2012AA101105)
河南省教育厅攻关项目(14B210022)
