摘要
目的探讨大鼠的最佳抗凝比,分析标本血量不足对凝血四项检测结果的影响。方法 60只大鼠分成2组,按真空采血法采集空腹12 h腹主动脉血液。第一组20只,用于全血细胞测定。全自动血液细胞计数仪检测红细胞压积(hematocrit,HCT),血小板计数(platelet,PLT)。第二组40只,每只鼠采2管血,按抗凝比[枸橼酸钠抗凝剂与全血的比例(V∶V)]分成1∶9(对照组)及1∶5(实验组),1∶8(实验组)及1∶7(实验组),离心获乏血小板血浆。全自动血凝分析仪检测凝血四项:凝血酶原时间(prothrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplatin time,APTT)、凝血酶时间(thrombin time,TT)、纤维蛋白原(Fibrinogen,FIB)。结果 SD大鼠HCT(%)为41.7±2.9,PLT(×109/L)为1114±173。随着抗凝比的增大,PT、APTT、TT延长,FIB减小。与对照组相比:1∶8实验组,差异无统计学意义。1∶7实验组,除TT差异有统计学意义外,另3项指标差异无统计学意义。1∶5实验组,差异有统计学意义。结论凝血四项的结果受抗凝比的影响。1∶9是大鼠最佳抗凝比,1∶8尚可。大鼠有其独特的生理特性。为大鼠的相关研究提供了基础的科学数据和基本的理论依据。
Objective To explore the best anticoagulant ratio in SD rats. To analyse the influence of insufficient blood specimens volume on coagulation tests. Methods 60 rats were divided into 2 groups. According to the method of vacuum blood,collect abdominal aortic blood after fasting 12 hours. The first group 20 rats were used only for routine blood test. Fully automatic hematology analyzer detected hematocrit and platelet. The second group 40 rats were used for coagulation test. Every rat was collected 2 blood specimens with different anticoagulant ratio [the proportion of sodium citrate anticoagulation and the whole blood（ vlume∶ volume） ]1∶ 9（ the control group） and 1∶ 5（ the experimental group）,1∶8（ the experimental group） and 1∶ 7（ the experimental group）. Get plasma without platelet through centrifugation. Fully automatic blood coagulation analyzer detected prothrombin time,activated partial thromboplatin time,thrombin time and Fibrinogen. Results HCT（ %） and PLT（ x 10^9/ L） in SD rats were respectively 41. 7 ± 2. 9 and 1114 ± 173. As anticoagulant ratio was increased,PT,APTT and TT were extended and FIB was decreased. Compared with the control group,these PT,APTT,TT,FIB four results of 1 ∶ 8 group were not statistically different,of 1 ∶ 5 group were statistically different,these PT,APTT,FIB three results of 1∶ 7 group were not statistically different,TT of 1∶ 7 group was statistically different. Conclusions The detection of coagulation project coagulation test results were affected by the proportion of anticoagulant and blood. 1∶ 9 was the best anticoagulant ratio in SD rats,1 ∶ 8 can also. Rat had its unique physiological characteristics. The results could provide reference for the evaluation of rats.
出处
《中国比较医学杂志》
CAS
北大核心
2015年第10期42-45,共4页
Chinese Journal of Comparative Medicine
