摘要
建立了高效液相色谱同时检测枸杞中槲皮素、山柰酚和异鼠李素的分析方法。样品经过甲醇超声提取后,用甲醇-25%HCl水解1 h,采用Inertsustain C18色谱柱进行分离,以甲醇-0.4%H3PO4溶液（48∶52,V/V）为流动相,进行等度洗脱,流速为1.0 m L/min,二极管阵列检测器检测,检测波长为360 nm,柱温为40℃。槲皮素,山柰酚,异鼠李素在40 min内实现分离,并分别在0.053~21.2μg/m L,0.053~4.24μg/m L和0.046~3.72μg/m L范围内具有良好的线性关系,相关系数为0.9972~0.9992,测得槲皮素、山柰酚、异鼠李素的加标回收率为99.2%~103.1%,95.6%~101.8%,93.2%~109.1%;相对标准偏差分别为0.95%~2.8%,0.55%~2.3%,0.81%~2.4%。对槲皮素、山柰酚和异鼠李素的检出限分别为0.04,0.05,0.03 mg/kg。方法可用来测定枸杞中3种黄酮苷元的含量。
A high performance liquid chromatographic method was established for simultaneous determination of quercetin,kaempferol and isorhamnetin in Lycium barbarum L. The samples were extracted by methanol and hydrolyzed by methanol- 25% hydrochloric acid（ 4： 1） for 1 h,then the separation was preformed on an inertsustain C18（ 250 mm × 4. 6 mm,5 μm） column,with methanol-0. 4% phosphoric acid as mobile phase（ 48： 52） and using isocratic elution at a flow rate of 1. 0 m L/min. The photodiode array detector was set at360 nm. The column temperature was 40℃. The results indicated that three substances were completely separated in 40 min. The calibration curves showed good linearity in the ranges of 0. 053 ~ 21. 2 μg/m L for quercetin,0. 053 ~ 4. 24 μg/m L for kaempferol,0. 0465 ~ 3. 72 μg/m L for isorhamnetin,with correlation coefficients of 0. 9972 ~ 0. 9992. The recoveries of three substances were in the range of 99. 2% ~ 103. 1%,95. 6% ~ 101. 8%,93. 2% ~ 109. 1%,with relative standard deviations（ RSD,n = 5） of 0. 95% ~ 2. 8%,0. 55% ~ 2. 4%,and 0. 81% ~ 2. 4%. The limits of detection for quercetin,kaempferol,and isorhamnetin（ LOD,S /N = 3） in Lycium barbarum L. were in the ranges of 0. 04,0. 05,and 0. 03 mg /kg,respectively.This method was simple,rapid and sensitive,and was applied in the determination of three flavonoid aglycones in Lycium barbarum L.
出处
《分析试验室》
CAS
CSCD
北大核心
2015年第11期1336-1338,共3页
Chinese Journal of Analysis Laboratory
关键词
枸杞
槲皮素
山柰酚
异鼠李素
高效液相色谱
Lycium barbarum L.
Quercetin
Kaempferol
Isorhamnetin
High performance liquid chromatography
