摘要
在粳稻品种Dongjin大田种植过程中,发现一个黄绿叶自然突变体,命名为djyg。该突变体在苗期表现明显的黄绿叶表型,抽穗以后,叶色逐渐恢复正常。叶绿素含量测定结果表明,在苗期、分蘖盛期及抽穗期叶绿素b的含量分别下降53%、62%、36%。电镜结果表明,分蘖期突变体中基粒、类囊体垛堆凌乱、排列疏松,类囊体基质较为稀薄。qRT-PCR结果证实,PORA、Cab1R、PsbA的表达量在突变体中均较野生型明显下调。遗传分析结果表明,黄绿叶突变体djyg由一对隐性主效核基因控制,图位克隆确定该候选基因为编码叶绿素合成酶基因YGL1的一个新等位基因。该突变体未影响植株的主要农艺性状,可作为一个理想的表型标记应用于杂交稻育种工作中。
A spontaneous yellow-green mutant was obtained from the japonica rice cultivar Dongjin in the field,designated as djyg.The mutant showed obvious yellow-green leaf phenotype at the seedling stage but the chlorotic leaves returned to green after the heading stage.Consistent with the phenotype,the chlorophyll b content of the mutant was reduced by 53%,62% and 36% at the seedling,tillering and heading stages,respectively,compared with that of the wild type（WT）.At the tillering stage,we observed that the granas and thylakoid stacks displayed a mess and loose structure,as well as less density in the mutant djygcompared with the WT under a transmission electron microscopy.The transcriptional levels of PORA,Cab1 R,PsbA were also decreased in the mutant djyg.Genetic analysis and map-based cloning demonstrated that the phenotype of the mutant was controlled by a major recessive nuclear gene,which is an allele of chlorophyll synthase YGL1.The mutant djygcan be used as a phenotypic marker in rice breeding program since the main agronomic traits of the mutant djygare normal compared with those of the WT.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2015年第6期601-609,共9页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(31371928)
教育部"创新团队发展计划"资助项目(IRT1239)
湖南省高校科技创新团队项目(2012)
关键词
水稻
黄绿叶
突变体
图位克隆
Oryza sativa L.
yellow-green leaf
mutant
map-based cloning
