摘要
以栗生垫壳孢(Coniella castaneicola)为试材,研究了该菌在不同培养基、温度、pH值、光照和碳氮源条件下的生长特性,并测定7种杀菌剂对其毒力强弱。结果表明:AEA培养基最适合该菌生长,菌丝生长最适温度为28℃、最适pH 5.0,不同光照条件对菌丝生长影响较小,最佳碳源和氮源分别是甘露醇和NH4NO3;7种杀菌剂对其毒力表现为氟环唑最强,丙森锌最弱;EC50分别为0.071 9、0.087 6、0.185 7、0.445 0、0.656 9、0.741 2、78.638 4μg/mL。
Taking Coniella castaneicolaas material,growth performance of the fungus under different media,temperatures,pH value,light periods,carbon and nitrogen sources were tested,and toxicity of seven fungicides to C.castaneicola was determined.The results showed that the optimal medium,temperature,pH value,carbon source and nitrogen source for the mycelial growth of the fungus was AEA,28℃,5.0,Mannitol and NH4NO3,respectively.However,light duration had no evident impact on the mycelial growth.The results also showed that Epoxiconazol had the highest toxicity,and the propineb was the lowest and the EC50 value was 0.071 9,0.087 6,0.185 7,0.445 0,0.656 9,0.741 2,78.638 4μg/mL,respectively.The results had laid good foundation for further studying the occurrence and control of grape white rot.
出处
《北方园艺》
CAS
北大核心
2015年第22期121-124,共4页
Northern Horticulture
基金
国家自然科学基金资助项目(31160358)
关键词
栗生垫壳孢
葡萄白腐病
生物学特性
毒力测定
Coniella castaneicola
grape white rot
biological characteristics
toxicity test
