摘要
采用组织匀浆法对枸杞各药用组织内生真菌和放线菌进行分离,共得到29株,真菌18株、内生放线菌11株,内生菌的数量在各组织器官中表现为根皮>叶>花>果实;通过Fenton反应法和T-AOC法对其中11株内生菌的羟自由基清除能力和总抗氧化能力进行测定,结合培养特性与序列分析法对菌株进行分类归属,其中5株内生真菌隶属于3属,6株内生放线菌属于3科3属;抗氧化实验结果表明5株内生真菌与6株内生放线菌的总抗氧化能力和羟自由基清除能力差异较大,曲霉属菌株抗氧化活性相对较强,其中f L6总抗氧化能力为(188.5±0.549)U·m L-1,IC50.3 mg·L-1,f L1的IC500.42 mg·L-1,f L9总抗氧化能力为(113.63±1.021)U·m L-1,都强于阳性对照Vit C;表明宁夏枸杞内生真菌具有潜在的抗氧化药物开发应用前景。
In this paper,29 endophytes were isolated from different organs and tissues of Lycium barbarum of Ningxia by tablet coating method,18 of them was fungi,and 11 of them was actinomycetes. The endophytes quantity in the different tissues were leaves flowers roots fruits; The hydroxyl radical scavenging activities of 11 endophytes were investigated by Fenton reaction,and total antioxidant capacities of them were examined by a total antioxidant capacity test kit; culture features and strain-specific sequence analysis were employed to explore the diversity of the 11 endophytes. The result showed that 5 fungi and 6 actinomycetes that having antioxidant activity could be phylogenetically classified into 3 genera,3 genera and 3 families,respectively. The total antioxidant capacity and hydroxyl radical scavenging activity of the 11 endophytes showed distinct difference. The antioxidant activity of Aspergillus were stronger,among which total antioxidant capacity of f L1 was（ 188. 5 ± 0. 549） U·m L^-1and the IC50 was 0. 3 mg·L^-1; the IC50 of strain f L1 was 0. 42 mg·L^-1and the total antioxidant capacity of f L9 was（ 113. 63 ± 1. 021） U·m L^-1,all of them were stronger than the positive control Vit C. The experimental results indicated that endophytic fungi of L. barbarum of Ningxia have a great developing and application prospect for the development of antioxidant agent.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第20期3941-3944,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(31360361)
2012年宁夏高等学校科学研究项目
关键词
宁夏枸杞
内生菌
抗氧化
序列分析
Lycium barbarum
endophytes
antioxidant activity
sequence analysis
