摘要
目的观察复方人参五味子片及其中的单味药人参、五味子、丹参、麦冬对小鼠酒精性肝损伤细胞的保护作用。方法用无水乙醇作用小鼠原代肝细胞,制备酒精性肝细胞损伤模型。细胞换液时将药物溶液加入新鲜培养基中作用细胞。收集培养上清液,测定谷胱甘肽酶(GSH)值、丙二醛(MDA)值。结果:小鼠酒精性肝细胞损伤使MDA值升高、GSH酶活性降低。人参组、丹参组、麦冬组及复方组对小鼠酒精性肝细胞损伤GSH酶有升高作用;五味子、丹参、麦冬及复方组对MDA值有降低作用,均以复方组表现最好。结论人参五味子片对小鼠酒精性肝细胞损伤具有良好的保护作用。
Objective To observe the protective effects of the formula tablet of Ginseng root and Schisandra chinensis fruit drugs and its compositions, GINSENG RADIX ET RHIZOMA（GRR）,SCHISANDRAE CHINENSIS FRUCTUS（SCF）, SALVIAE MILTIORRHIZAE RADIX ET RHIZOMA（SMRR）, OPHIOPOGONIS RADIX（OR） on the alcohol-induced liver cell injury.Method： Alcohol-induced liver cell injury models were established with the rat liver cell cultured by the ethanol, and then therapeutic agents were given respectively. The contents of malonyl dialdehyde（MDA）, and reduced glutathione（GSH） in hepatic homogenate from all groups of liver cells were determined. Results： In alcohol treated liver cell suspension, MDA were increased markedly while GSH activity decreased significantly. It showed that GRR, SMRR, OR and the formula tablet had an effect on GSH; SCF, SMRR, OR and the formula tablet had a marked effect on MDA, and the formula tablet show the best effect. Conclusion The formula tablet had optimal effect on alcohol-induced liver cell injury.
出处
《人参研究》
2015年第3期8-9,共2页
Ginseng Research
基金
吉林省科技厅科技发展计划项目
项目编号:20140311036YY
关键词
人参等
配伍
保护
酒精性肝细胞损伤
Ginseng and others
Formula
Protective effect
Alcohol-induced liver cell injury
