摘要
1目的通过基因组特异PCR扩增鉴定MicroRNA-150基因敲除小鼠。2方法利用碱裂解法提取鼠尾基因组DNA,在小鼠MicroRNA-150基因两端设计特异引物,PCR扩增检测。3结果正常对照小鼠DNA经PCR扩增产生长度为866个碱基对的DNA片段,MicroRNA-150基因敲除小鼠产生长度为262个碱基对的DNA片段。4结论通过碱裂解法提取鼠尾DNA并采用特异引物扩增能够准确鉴定MicroRNA-150基因敲除小鼠。
Objective To identify microRNA-150 knockout mice（miR-150KO ）by genomic PCR amplification.Methods Mouse tail DNA was extracted by alkaline lysis,and amplified by PCR u- sing primers that designed at each end of the miR-150 gene.Resuits Wild type （WT） mice produced a 866 base pair DNA fragment,while miR-150KO mice produced a 262 base pair DNA fragment after mouse tail DNA PCR amplification.Conclusion MiR-150KO mice can be accurately identified by PCR amplification,which using alkaline lysis extracted genomic DNA and miR-150 gene specific primers.
出处
《河北联合大学学报（医学版）》
2015年第6期237-238,共2页
Journal of North China Coal Medical College
基金
华北理工大学大学生创新创业训练计划项目(编号:X2015153)
国家自然科学基金项目(编号:81373111)
河北省自然科学基金项目(编号:H2013209019)
