摘要
目的:用高效液相色谱法测定止痒洗剂中盐酸小檗碱含量。方法:采用Hedera C_(18)柱(250×4.6 mm,5μm),以乙腈-0.1%磷酸溶液(50∶50,每100ml加十二烷基磺酸钠0.1g)为流动相,流速为1.0ml·min^(-1),检测波长265nm。结果:盐酸小檗碱的检测浓度在3.8125~122.0μg·ml^(-1)范围内与峰面积积分值呈良好的线性关系(r=0.9999),平均加样回收率为100.70%,RSD=0.85%(n=6)。结论:HPLC法简便易行,结果准确,重现性好,可用于测定止痒洗剂中盐酸小檗碱含量。
Objective To establish a method for the determination of berberine hydrochloride in ZhiYang Lotion by HPLC.Meth-ods The Hedera C18 column （250 mm × 4.6 mm,5 μm）was used,acetonitrile -0.1% phosphoric acid solution （50∶50,0.1 g sodium dodecyl sulfonate add in each 100 ml solution）as the mobile phase.The flow rate was 1.0 ml·min -1 ,and the detection wavelength was 265 nm.Results The linear range of berberine hydrochloride was 0.8844 -28.3 μg·ml -1 （r =1.0000）,the aver-age recovery rate was 100.70%,and RSD was 0.85% （n =6）.Conclusion The method is simple,rapid,accurate and reliable.It can be used for the content determination of berberine hydrochloride in ZhiYang Lotion.
出处
《中国民族民间医药》
2015年第22期25-26,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy
