摘要
目的探讨新的减阻聚合物芦荟多糖较传统减阻聚合物聚环氧乙烷(PEO)对盲肠结扎-穿刺(CLP)脓毒症模型大鼠肾功能的保护作用。方法 64只SD雄性大鼠随机均分为四组:假手术+生理盐水组(S组)、CLP+生理盐水组(NS组)、CLP+聚环氧乙烷组(PEO组)和CLP+芦荟多糖组(Aloe组)。CLP造模后24h制备脊斜方肌微循环活体标本,随后分别给予生理盐水20ml/kg(S组和NS组)和等容积的芦荟多糖(PEO组和Aloe组)。记录给药前和给药后2h微动脉红细胞流速和管径的变化。每组取8只大鼠放回笼子观察48h存活时间,剩余8只大鼠24h采取安乐死法处死,取血标本检测肾功能和血清炎症因子水平,取肾脏组织行病理检查和评分。结果与NS组比较,PEO组和Aloe组微动脉红细胞流速提高,肾功能改善,炎症反应减轻,存活时间均明显延长(P<0.05或P<0.01)。但Aloe组大鼠血清尿素氮(Bun)、肌酐(Cr)和肾脏病理评分明显低于PEO组(P<0.05)。结论芦荟多糖减阻剂能够减轻CLP脓毒症大鼠肾损伤,其机制与改善微循环和抑制炎症反应有关。
Objective To investigate the protective effects of aloe polysaccharide as a new drag- reducing polymer on renal function in CLP rats model compared with the traditional drag-reducing pol- ymer polyethylene oxide （PEO）, and to provide information for the potential clinical application of drag-reducing polymers. Methods Sixty-four male SD rats were randomly divided into 4 groups： sham operation＋normal saline group（group S）, CLP-＋norrnal saline group （group NS）, CLP＋poly- ethylene oxide group （PEO） and CLP＋aloe polysaccharide group （group Aloe）, with 16 rats in each group. CLP model was induced and 24 h later, spinotrapezius muscle living specimens were prepared, followed by drug administration subsequently. Red blood cell velocity and diameter of arteriole were recorded before and after drug administration in the next 2 h. After that, some rats （n=8 in each group） were put back into the cages for 48 h survival observation, and the remaining rats （n=8 in each group） were killed 24 hours later. Indexes of renal function and serum levels of inflammatory factors were determined, and tissues of kidney were collected for pathological examination. Results Compared with group NS, velocities of blood red cell were increased in groups PEO and Al- oe, with improved renal function and reduced inflammatory response; also the survival time was sig- nificantly prolonged（P〈0. 05 or P〈0. 01）. Better renal protection effects were found in group Aloe than that in group PEO, which showed significantly improved renal function indexes （P〈0.05）. But the serum Bun and Cr and renal pathological score in group Aloe were significantly lower than that in group PEO （P〈0.05）. Conclusion Aloe polysaccharide drag-reducing polymer can reduce renal dam- age in CLP rats and its mechanisms are related with microcirculation improvement and inflammatory reaction inhibition.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2015年第11期1109-1112,共4页
Journal of Clinical Anesthesiology
基金
广东省科技计划项目(2011B061200027)
关键词
脓毒症
减阻聚合物
芦荟多糖
肾功能
微循环
Sepsis
Drag reduction polymers
Aloe polysaccharide
Renal function
Micro- circulation
