摘要
以番茄抗黄化曲叶病毒ty-5基因的抗病材料‘13072’(亚洲蔬菜研究与发展中心提供)和感病材料‘13493’(早粉2号)为亲本配置杂交组合,获得F1、F2、BC1P1和BC1P2 4个世代,对番茄黄化曲叶病抗性基因ty-5进行遗传规律分析和基因定位研究。结果表明,抗性基因ty-5为隐性基因控制。利用657株F2分离单株,应用群体分离分析(BSA)法筛选得到与ty-5基因连锁的5个多态性SSR标记,构建了ty-5基因的分子标记连锁图谱,将ty-5基因定位到番茄4号染色体上,物理距离为737 kb的区段内,两侧翼标记为TES2461和TGS4151,与ty-5遗传距离分别为2.4 c M和3.1 c M。生物信息学分析表明,该区段存在52个预测候选基因。
Genetic analysis and gene mapping were carried out on which is resistant to Tomato yellow leaf curl virus using tomato（Solanum lycopersicum L.）‘13072＇（resistant materials）and‘13493＇（susceptible materials）as experimental materials in this study. The results showed that a recessive nuclear gene,ty-5,controls the resistance to Tomato yellow leaf curl virus. Simple sequence repea（tSSR）markers were used to conduct on the resistant 657 F2 plants by bulked segregant analysis（BSA）method,and resulted in 5 SSR markers linked to the ty-5 gene. The ty-5 gene was mapped to a linkage group corresponding to chromosome（Chr. 4）of tomato. The flanking markers TES2461 and TGS4151 were linked to the ty-5 gene with genetic distances of 2.4 c M and 3.1 cM,respectively. The physical distance between markers TES2461 and TGS4151 was 737 kb based on the whole genome sequence including 52 candidate genes in this region.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第10期1965-1973,共9页
Acta Horticulturae Sinica
基金
国家科技支撑计划项目(2012BAD02B02-7)
国家自然科学基金项目(31272171)
黑龙江省杰出青年科学基金项目(JC201204)
关键词
番茄
番茄黄化曲叶病
遗传分析
分子标记
连锁图谱
tomato
Tomato yellow leaf curl virus
genetic analysis
SSR marker
gene mapping
