摘要
以茄子单性结实品系‘D-7-1’为材料,克隆到3个新的Aux/IAA基因,分别命名为Sm IAA3、SmIAA4和Sm IAA9,其CDS长度分别为573、564和915 bp,分别编码190、187和304个氨基酸;系统进化树分析表明,3个蛋白均属于Aux/IAA家族蛋白,与番茄亲缘关系较近,且均具有Aux/IAA家族蛋白的DomainⅠ、Ⅱ、Ⅲ和Ⅳ保守结构域;其分子量分别为21.50、20.95和32.67 k D,理论等电点分别为7.62、6.02和8.57。实时定量PCR分析表明:SmIAA9基因在单性结实和非单性结实品系茄子中的表达量差异明显,开花前单性结实品系的表达量显著高于非单性结实品系,推测其与茄子单性结实相关。
Three Aux/IAA genes Sm IAA3,Sm IAA4 and Sm IAA9 in eggplant were cloned from parthenocarpic eggplan‘tD-7-1＇. The full CDS length of Sm IAA3,Sm IAA4 and Sm IAA9 was 573,564 and 915 bp,which encoded three polypeptides of 190,187 and 304 amino acids. The phylogenetic analysis showed that the three proteins belonged to Aux/IAA family proteins and they had the highest similarity with Aux/IAA proteins of tomato. And the three novel proteins contained conserved domainⅠ,Ⅱ,Ⅲ and Ⅳ of Aux/IAA family proteins. The molecular weight of the three proteins were 21.50,20.95 and 32.67 k D with isoelectric point 7.62,6.02 and 8.57 respectively. The RT-PCR analysis showed that the expression of Sm IAA9 gene was different between parthenocarpic and nonparthenocarpic eggplant. Evidently the parthenocarpic was higher than the nonparthenocarpic before flowering,which indicated that Sm IAA9 would be a gene related with parthenocarpy.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第10期2049-2058,共10页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31501756)
国家现代农业产业技术体系建设专项资金项目(CARS-25-A-06)
中央高校基本科研业务费专项(XDJK2014C092)
