摘要
目的:检测并分析PPARδ基因SNP/rs2267668的A/A基因型的杰出有氧耐力表型与训练诱导的循环microRNA(c-miRNA)表达特征反映的microRNA(miRNA)调控特征的关联。方法:运用递增负荷法检测33名现役国家级耐力项目运动员和58名体育专业大学生(二级运动员)的最大摄氧量、个体乳酸阈,同步采用microarray及qRT-PCR法检测训练诱导的c-miRNA表达谱,Taqman法解析PPARδ基因SNP/rs2267668基因型,分析优势基因型及其杰出耐力表型与训练诱导的miRNA调控特征的关联。结果:A/A基因型的杰出耐力表型差异表达17条c-miRNA,9条上调,8条下调,其靶基因富集于AMPK、p53、PPAR、MAPK、m TOR信号通路,miRNA的调控作用使AMPK-e NOS通路和p53通路协同上调,其调控特征与rs2267668引起的PPARδ基因功能变化有关联,对杰出耐力表型的形成具有促进作用。结论:rs2267668的A/A基因型的杰出有氧耐力表型与训练诱导的循环miRNA差异表达特征存在关联,两者可作为预测有氧耐力发展潜力的表观遗传学分子标记联合应用于运动选材。
Objective： to explore the correlations between the elite endurance phenotype of A / A genotype of rs2267668 and the differential expression profile of c- miRNA induced by exercise.Methods： 33 active elite athletes（ national grade） and 58 student athletes（ grade 2） were engaged in this test. VO2 m axand ILT were measured. Rs2267668 and differential expression profile of c-miRNAs induced by exercise were detected. Targetscan,mrRanda,and miRBase were used to predict target genes of those miRNA,and pathway analysis was performed for target gene by KEGG and IPA. Results： There were 17 c- miRNAs which regulated the pathway of AMPK,p53,PPAR,MAPK,m TOR,and their regulating characters were related to the function of PPAPδ enhanced by A / A genotype,which were validated to promote the formation of the elite endurance. Conclusion： Cooperated with rs2267668,the profile of c-miRNAs induced by exercise can be used as a biomarker on forecasting and valuing the potential of aerobic endurance.
出处
《上海体育学院学报》
CSSCI
北大核心
2015年第6期76-83,共8页
Journal of Shanghai University of Sport
基金
国家科技部攻关项目(2005BA904)
