摘要
为了探明松材线虫(Bursaphelenchus xylophilus)与拟松材线虫(Bursaphelenchus mucronatus)能否进行种间杂交,收集了不同地理来源的松材线虫和拟松材线虫株系,在实验室内进行了种间杂交、继代培养以及回交。结果显示:中国浙江松材线虫和法国拟松材线虫、中国浙江松材线虫和中国拟松材线虫杂交组合,具有较高的交配率、繁殖量和低死亡率。在继代培养试验中,这2个组合都具有很强的繁殖优势,其杂交后代可以同亲本回交,且更趋于同拟松材线虫回交。总的来说,松材线虫和拟松材线虫有些虫株间是可以杂交并能够顺利繁殖,建立可延续的种群的。推测在自然条件下,松材线虫和拟松材线虫杂交后代是存在的。
In our study different isolates of Bursaphelenchus xylophilus and B. mucronatus were collected from different origins.Interspecific hybridization,successive transfer culture and backcross test were conducted in the laboratory. The combinations of B. xylophilus from Zhejiang hybridizes and B. mucronatus from France or B. mucronatus from China had high hybrid rate,large number of descendants,and low death rate. In subculture tests,both combinations had a strong reproductive advantage. The hybrid offspring of two combinations could cross with parents,especially the B. mucronatus. Therefore,there is interspecific hybridization between some B. xylophilus and B. mucronatus actually. The hybrids can establish stable population,and there is the hybrid offspring of B. xylophilus and B. mucronatus exist in natural conditions.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2015年第11期103-105,共3页
Journal of Northeast Forestry University
基金
国家林业公益性行业科研专项(201204501)
国家自然科学基金项目(31170600)
关键词
松材线虫
拟松材线虫
杂交
繁殖力
Bursaphelenchus xylophilus
Bursaphelenchus mucronatus
Hybrid
Fecundity
