摘要
目的：观察小儿清瘟解热颗粒对甲型H1N1流感病毒感染小鼠的影响。观察小儿清瘟解热颗粒体外抗病毒作用。方法：ICR小鼠随机分为7组,分别为正常组、模型组、利巴韦林组（82.5 mg·kg^-1）,小儿感冒宁合剂组,小儿清瘟解热颗粒高、中、低剂量组（12.8,6.4,3.2 g·kg^-1）,每组10~12只,体内抗病毒方面,以甲型H1N1流感病毒滴鼻感染ICR小鼠造成肺炎模型,造模同时开始给予小儿清瘟解热颗粒12.8,6.4,3.2 g·kg^-1ig,以利巴韦林为阳性药,观察肺部炎症,计算肺指数,死亡率和生命延长率,酶联免疫法检测血清中细胞因子肿瘤坏死因子-α（TNF-α）,一氧化氮（NO）,γ干扰素（IFN-γ）的含量;体外抗病毒方面,采用CEP法观察病毒致细胞病变情况。结果：与模型组比较,小儿清瘟解热颗粒治疗性给药后能明显降低流感病毒FM1株感染后小鼠的肺指数,降低死亡率,延长平均存活天数;小儿清瘟解热颗粒中、低剂量组可明显升高感染小鼠血清中IFN-γ,NO含量（P〈0.05,P〈0.01）。体外抗病毒方面,小儿清瘟解热颗粒可明显抑制甲型H1N1流感病毒（FM1株,PR8株,brisbane/59/2007株）,甲型H3N2型流感病毒（brisbane/10/2009株）,乙型流感病毒（江西修水/32/2009株）,副流感病毒1型（PIV-1）,柯萨奇病毒B族2型（Cox B4）致细胞的病变作用。结论：小儿清瘟解热颗粒对甲型流感病毒感染小鼠造成的病毒性肺炎具有明显的治疗作用,对体外多种病毒也有一定程度的抑制作用。
Objective： To observe the effect of Xiaoer Qingwen Jiere granules on the mice models infected by influenza A（ H1N1）. To observe the in vitro antiviral effect of Xiaoer Qingwen Jiere granules.Method： ICR mice were randomly divided into 7 groups,including normal group,model group,Ribavirin group（ 82. 5 mg·kg- 1）,Xiaoer Ganmaoning mixture group,Xiaoer Qingwen Jiere granules high,middle,low dose groups（ 12. 8,6. 4,3. 2 g·kg- 1）,n = 10-12 in each group. In respect of in vivo antiviral aspect,ICR mice infected by influenza A（ H1N1） were used as pneumonia models. Xiaoer Qingwen Jiere granules（ 12. 8,6. 4,3. 2g·kg- 1） were given since the time of modeling,with Ribavirin as the positive drug to observe the lung inflammation,calculate lung index,death rate,and life prolongation rate. Serum cytokines tumor necrosis factor α（ TNF-α）,nitric oxide（ NO）,and γ interferon（ IFN-γ） were detected by ELISA. In respect of in vitro antiviral aspect,CEP method was used to observe the pathological changes of virus. Result： Compared with the model group,Xiaoer Qingwen Jiere granules could significantly decrease the lung index and death rate of ICR mice and prolong their life span. Xiaoer Qingwen Jiere granules middle and low dose groups could significantly increase serum INF-γ,and NO levels in infected mice（ P〈0. 05,P〈0. 01）. In respect of in vitro antiviral aspect,Xiaoer Qingwen Jiere granules can significantly suppress pathological changes by H1N1 influenza A virus（ FM1 strain,PR8 strain,Brisbane /59 /2007）,H3N2 influenza A virus（ Brisbane /10 /2009 strain）,influenza B virus（ jiangxi xiushui /32 /2009 strain）,parainfluenza virus 1（ PIV-1）,and coxsackie virus B type 4（ Cox B4）. Conclusion：Xiaoer Qingwen Jiere granules has obvious therapeutic effect for the viral pneumonia caused by influenza A virus in mice,and also has certain inhibition effect on multiple viruses in vitro.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第22期128-132,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
北京市科学技术委员会"十病十药"研发项目(Z111102059011003)
关键词
小儿清瘟解热颗粒
流感病毒
病毒性肺炎
Xiaoer Qingwen Jiere granules
influenza virus
viral pneumonia
