摘要
目的通过观察正天丸对戊巴比妥钠及巴比妥钠催眠作用的影响,了解正天丸对肝微粒体细胞色素P450活性的影响。方法将60只小鼠随机分为溶媒组(2%吐温-80,20 ml/kg)、氯霉素组(25 mg/kg)及正天丸低、高剂量组(3,6 g/kg),每组15只,连续等容灌胃3d,末次给药1h后,再给小鼠腹腔注射戊巴比妥钠40 mg/kg或巴比妥钠180 mg/kg,观察小鼠的睡眠潜伏期和睡眠时间。结果氯霉素组和低、高剂量的正天丸组使戊巴比妥钠对小鼠的催眠时间分别延长222.2%、123.1%、42.6%,其中氯霉素组和小剂量正天丸组与溶媒组比较,小鼠的睡眠时间显著延长,均P<0.01。与溶媒组比较,高剂量的正天丸能显著延长巴比妥钠催眠潜伏期(P﹤0.01),并缩短催眠时间(P﹤0.01)。结论正天丸可能抑制小鼠肝微粒体细胞色素P450活性,同时对中枢神经系统有兴奋作用。
Objective：To observe the influence of Zheng-tian pill on the hypnotic effects of pentobarbital sodium and barbital sodium. Methods：Mice were randomly divided into solvent（5% Tween-80,20 ml/kg）,chloromycetin（25 mg/kg）and Zheng-tian pill（3,6 g/kg）groups,which were administered orally with equivalent volume,once a day and for three days. An hour later at the last administration,all the animals were given pentobarbital sodium（40 mg/kg,ip）or bar-bital sodium（180 mg/kg,ip）,and then the latent period and duration of hypnosis were observed. Results：Hypnotic dura-tion of pentobarbital sodium was prolonged by 222. 2%,123. 1% and 42. 6% in chloromycetin and two Zheng-tian pill groups respectively,chloromycetin group and Zheng-tian pill group at the lower dose compared with solvent group,there were evident difference（P﹤0. 01）,and yet the large dose of Zheng-tian pill prolonged evidently hypnotic latent period（P﹤0. 01）and shortened obviously hypnotic duration（P﹤0. 01）of barbital sodium. Conclusion：The findings in this study suggest that Zheng-tian pill is likely to inhibit liver microsomal cytochrome P450 activity and to excite the central nervous system at a higher dose in mice.
出处
《泰山医学院学报》
CAS
2015年第9期982-984,共3页
Journal of Taishan Medical College
