期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

AQP4及AQP4 mRNA在重型脑损伤中脑脊液中的表达及意义

下载PDF
导出
摘要 目的观察重型脑损伤后脑脊液中水通道蛋白(4AQP4)及AQP4mRNA的表达变化及意义。方法选取重型颅脑损伤患者51例,对每例患者第0、3、7天脑脊液进行AQP4ELIS(A酶联反应吸附实验)含量测定,RT-PC(R实时定量聚合酶链式反应)测定AQP4mRNA表达。结果第0、3、7天脑脊液中均可检测AQP4及AQP4mRNA。第3天较第0天升高(P<0.05),第7天较第3天降低(P<0.05),第7天与第0天比较,差异无统计学意义。AQP4与AQP4mRNA相关(r=0.867)。结论重型脑损伤后患者脑脊液AQP4的表达随着病程变化,推测AQP4可能参与了脑损伤的病理过程,与病情的转归相关。 Objective To observe the change of aquaporin (AQP)4 and AQP4 mRNA level in the cerebrospinal fluid of patients with severe brain injury and its clinical significance.Methods There are 51 severe traumatic brain injury patients. ELISA (enzyme-linked immunosorbent assay) was used to determine cerebrospinalfluid AQP4 protein expression after 0,3,7 days,and RT-PCR (reverse transcription PCR) was used to determine AQP4 mRNA expression.Results AQP4 protein or mRNA expressed within the 0,3,7 days.The level of AQP4 or AQP4 mRNA increased 3 days after operation (P <0.05),and then statistically decreased 7 days after operation (P <0.05). But the 7 days had no statistically difference compared with 0 day after operation.Conclusion After Brain injury, AQP4 expression changes after brain injury is closely relatedwith the formation of cerebral edema, AQP4 in the damage brain edema formation plays an important role.
作者 谯明 彭艳 杨汉兵 雷振富 王俊英 彭雪刚 文刚
机构地区 眉山市第二人民医院(仁寿县人民医院)重症医学科 眉山市第二人民医院(仁寿县人民医院)神经外科 眉山市第二人民医院(仁寿县人民医院)检验科
出处 《当代医学》 2015年第34期16-18,共3页 Contemporary Medicine
关键词 重型脑损伤 水通道蛋白4 Aevere brain injury Aquaporin 4
分类号 R651.15 [医药卫生—外科学]
  • 相关文献

参考文献11

  • 1Agre P,Bonhivers M,Borgnia MJ.The aquaporins,blueprints for cellularplumbing 9ystems[J].J Biol Ghem, 1 998,273(24).. 14659-14562 .
  • 2Tang Y,Wu P,Su J,et al.Effects of Aquaporin-4 on edema formationfollowing intracerebral hemorrhage[J], Hxp Neurol, 201 0, 223(2). 485-495.
  • 3Graber DJ, Levy M,Kerr D,et al .Neuromyelitis optica pathogenesis andaquaporin 4[J], J Neuroinflammationm, 2008,29(5); 22 .
  • 4Fukuda AM, Badaut J. Aquaporin 4 ; a player in cerebral edema andneuroinflammation[J].J Neuroinflammation,2012,9.279.
  • 5Ren ZJIiff JJ, Yang L,et al. 1 Hit & Run1 model of closed-skuiltraumatic brain injury(TBl)leveals complex patterns of post-traumaticAQP 4 dysregulation[J]. J Cereb Blood Flow Metab, 201 3, 33(6); 834-845.
  • 6Yao X,Hrabetov a S,Nicholson C,et al. Aquaporin-4-deficient micehave increased extracellular space without tortuosity change[J].JNeurosci,2008,28(21): 5460-5464.
  • 7翁晓建,李守缄,唐霍全.甘露醇对重型颅脑损伤后脑组织含水量及AQP4表达的变化[J].中国现代医生,2009,47(35):32-33. 被引量：2
  • 8Ding Z,Zhang J,Xu J, et al. Propofo1 administration modulates AQP-4expression and brain edema after traumatic brain injury[J].Gell BiochemBiophy,2013,67(2):615-622.
  • 9Chen GS,Huang KF,Huang CC’et al.Thaliporphine derivative improvesacute lung injury after traumatic brain injury[J]? Biomed Reslni;,2015,2015:729831 .
  • 10Tang G,Liu 丫,Zhang Z,et al .Mesenchymal stem cells maintain blood-brain barrier integrity by inhibiting aquaporin-4 upreguiation aftercerebral ischemia[J].Stem Ceils, 2014, 32(12). 31 50-3 J 62.

二级参考文献10

  • 1Kiening KL, Frank KH, Landeghem V, et al. Dereased hemispheric aquaporin-4 is linked to evolving to brain edema following controlled cortical impact injury incat[J]. Netrrosci Lett, 2002,342(2 ) : 105-108.
  • 2Davis M, Lucatorto M. Mannitol revisited[J]. J Neurosci Nurs, 1994,26 (2):170-174.
  • 3Winkler SR, Munoz-Ruiz L. Mechanism of action of mannitol [J]. Surg Neurol, 1995,43 ( 5 ) : 59-67.
  • 4Polderman KH, van de Kraats G, Dixon JM, et al. Increases in spinal fluid osmalarity induced by mannitol[J]. Crit Care Med, 2003,31 (2) : 584- 590.
  • 5Zelenina M, Zelenin S, Bondar AA, et al. Water permeability of Aquaporin-4 is decreased by protein kinase C and dopamine[J]. Am J Physiol Renal Physiol, 2002,283 : F309-318.
  • 6Dawson VL, Kizushi VW, Huang PL, et al. Resistance to neurotoxicity in cortical cultures from neuronal nitric oxide synthase-deficient mice[J]. J Neurosci, 1996,16 (4) : 2479-2487.
  • 7Chen J,Jin K,Chen M,et al. Early detection of DNA strand breaks in the brain after transient focal ischemia: implication for the role of DNA damage in apoptosis and neuronal cell death[J]. J Neurochem, 1997,69( 11 ): 232-245.
  • 8Dalkara T, Moskowitz MA. Neurotoxic and neuropmtective roles of nitric oxide in cerebral ischemia. In:Green AR, Cross A J, eds. Neuroprotective agents and cerebral ischemia[M]. San Diego:Academic Press, Inc, 1997 : 319-336.
  • 9Papadopoulos MC,Krishna S,Verkman AS,et al. Aquaporin water channels and brain edema[J]. Mt Sinai J Med, 2002,69 (17):242-248.
  • 10Papadopoulos MC, Manley GT, Krishna S, et al. Aquaporin-4 facilitates reabsorption of excess fluid in vasogenic brain edema[J]. FASEBJ, 2004, 18(2) : 1291-1293.

共引文献1

当代医学
2015年 第34期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部