摘要
[目的]提取白花蛇舌草总黄酮,探讨白花蛇舌草总黄酮对BGC-823胃癌细胞周期和凋亡的影响。[方法]用乙醇回流法提取白花蛇舌草总黄酮,标准曲线法测定其含量,AB-8大孔吸附树脂进行纯化,然后作用于BGC-823肿瘤细胞,流式细胞仪检测FHD对细胞周期及细胞凋亡的影响。[结果]乙醇回流法提取白花蛇舌草总黄酮,含量为67.2mg/g(总黄酮/干浸膏),纯度为6.72%。AB-8大孔吸附树脂纯化白花蛇舌草总黄酮后所得精制品中总黄酮纯度约为23.82%,纯化后的总黄酮纯度提高约4倍。白花蛇舌草总黄酮组较对照组G1期细胞增加,S期细胞明显减少;实验组凋亡细胞比例明显高于对照组。[结论]AB-8大孔吸附树脂对白花蛇舌草中总黄酮具有很好的纯化富集作用;白花蛇舌草总黄酮对胃癌细胞BGC-823株的生长有明显的抑制作用,作用机制可能与抑制DNA的合成,诱导细胞凋亡有关。
[Objective]To extract flavonoids of Hedyotis Hiffusa Wild（FHD）,and to explore the effect of FHD on gastric cancer cell BGC-823 in proliferation cycle and apoptosis. [Methods]FHD was extracted with ethanol reflux method. Standard curve method was used to determine the content,and AB-8 macro- porous adsorption resin was used for purification. Flow cytometry was used to detect proliferation cycle and apoptosis. [Results]FHD content extracted by ethanol reflux was 67.2 mg/g（total flavonoids/dry ex- tractum）. The purity was 6. 72%. After purified by AB-8 macroporous adsorption resin, the purity was 23. 82%. Compared to control group,cells in G1 phase of FHD increased,and cells in S phase decreased sig- nificantly; the apoptosis cell ratio was significantly higher than the control group. [Conclusion]AB-8 macro- porous adsorption resin is suitable for purification and enrichment of FH. FHD has inhibitory effect on gastric cancer cells BGC-823 in vitro. The mechanism may be related to inhibition of FHD in DNA synthe- sis and inducing cell apoptosis.
出处
《中国中西医结合消化杂志》
CAS
2015年第11期760-763,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
甘肃省高等学校基本科研业务费项目(No:BH2011-070)
甘肃省自然科学基金项目(No:145RJZA235)
