摘要
目的探讨不同浓度奥沙利铂对人视网膜母细胞瘤(RB)细胞Y79增殖及凋亡的影响。方法将Y79细胞于RPMI 1640培养基中进行培养,分别给予0.000、0.125、0.250、0.500、1.000、2.000μmol·L-1奥沙利铂对Y79细胞进行处理,检测Y79细胞增殖情况、凋亡情况及半胱氨酸天冬氨酸蛋白酶3(Caspase-3)活性变化。结果不同浓度的奥沙利铂对Y79细胞增殖均有抑制作用,且随着浓度的增加,抑制作用逐渐增强(P<0.05);与0.000μmol·L奥沙利铂比较,0.250μmol·L-1奥沙利铂对Y79细胞增殖已有较强的抑制作用(P<0.05)。0.000、0.250μmol·L-1奥沙利铂处理下Y79细胞凋亡率分别为(2.18±0.43)%、(36.94±1.36)%,0.250μmol·L-1奥沙利铂处理下Y79细胞凋亡率显著高于0.000μmol·L-1奥沙利铂处理时(P<0.01)。0.000、0.250μmol·L-1奥沙利铂处理下Y79细胞中Caspase-3活性分别为(1.97±0.72)%、(5.82±0.64)%,0.250μmol·L-1奥沙利铂处理下Y79细胞中Caspase-3活性显著高于0.000μmol·L-1奥沙利铂处理时(P<0.01)。结论奥沙利铂可抑制人RB细胞增殖,并促进其凋亡。
Objective To investigate the effect of different concentrations of oxaliplatin on human retinoblastoma（ RB）Y79 cells proliferation and apoptosis. Methods Y79 cells cultured in RPMI 1640 medium were dealt with 0. 000,0. 125,0. 250,0. 500,1. 000 and 2. 000 μmol · L- 1oxaliplatin. The Y79 cells proliferation,apoptosis and Caspases-3 activity were detected. Results Different concentrations of oxaliplatin had inhibitory effect on Y79 cell proliferation,and the inhibitory effect was enhanced with the increasing of concentration of oxaliplatin（ P〈0. 05）. Compared with 0. 000 μmol·L- 1oxaliplatin,0. 250 μmol·L- 1oxaliplatin had stronger inhibitory effect on Y79 cell proliferation（ P〈0. 05）. The apoptosis rate of Y79 cell dealt with 0. 000,0. 250 μmol·L- 1oxaliplatin was（ 2. 18 ± 0. 43） % and（ 36. 94 ± 1. 36） % respectively. The apoptosis rate of Y79 cell dealt with 0. 250 μmol · L- 1oxaliplatin was significantly higher than that dealt with0. 000 μmol·L- 1oxaliplatin（ P〈0. 01）. The activity of Caspases-3 in Y79 cell dealt with 0. 000,0. 250 μmol·L- 1oxaliplatin was（ 1. 97 ± 0. 72） % and（ 5. 82 ± 0. 64） % respectively. The activity of Caspases-3 in Y79 cell dealt with0. 250 μmol·L- 1oxaliplatin was significantly higher than that dealt with 0. 000 μmol·L- 1oxaliplatin（ P〈0. 01）. Conclusion Oxaliplatin can inhibit RB cell proliferation and promote the apoptosis.
出处
《新乡医学院学报》
CAS
2015年第11期989-991,共3页
Journal of Xinxiang Medical University
基金
陕西省科学技术研究发展计划资助项目(编号:2014K11-01-01-23)
