摘要
以葡萄品种赤霞珠(Cabernet Sauvignon)不同发育时期的果实为材料,研究葡萄总酚(TP)、总类黄酮(TF)、总黄烷-3-醇(TFO)、总花色苷(Tacy)、白藜芦醇(Res)含量与其相关酶活性的关系,为其酚类物质代谢调控提供依据。结果表明,果皮中总酚和白藜芦醇含量的变化呈极显著正相关,同时白藜芦醇含量与苯丙氨酸解氨酶(PAL)活性也呈极显著正相关。此外,总黄烷-3-醇与白藜芦醇和花色苷之间的累积相互抑制。推测苯丙氨酸解氨酶是白藜芦醇合成代谢调控的关键酶,总黄烷-3-醇可能参与白藜芦醇和花色苷之间的相互转换。
The berries of Vitis vinifera cv. Cabernet Sauvignon was used as material to investigate the correlation between the content of grape total phenolic（ TP）,total flavinoids（ TF）,total flavan-3-ol（ TFO）,total anthocyanim（ Tacy）,resveratrol（ Res） and the related enzymes activities with the aim to provide the theoretical basis for metabolism of phenolic compounds. The result showed that there was significantly positive correlation between the content of TP and Res. Simultaneously,a significantly positive correlation was observed between the content of Res and PAL activity. In addition,there were interactions of reciprocal inhibition between TFO and Res or Tacy,respectively.Therefore,PAL was confirmed as a key enzyme in the pathway of resveratrol metabolism regulation,and TFO might be involved in the conversion between Res and Tacy.
出处
《浙江农业学报》
CSCD
北大核心
2015年第11期1922-1926,共5页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(31260456)
关键词
葡萄
赤霞珠
酚类物质
酶活性
grape
Cabernet Sauvignon
phenolic compound
enzyme activity
