摘要
目的建立测定沙枣中槲皮素和异鼠李素含量的高效液相色谱法（HPLC法）。方法色谱条件：C18色谱柱,甲醇-0.1%磷酸溶液（58∶42）,检测波长370 nm。结果槲皮素在0.016~0.032μg范围内线性关系良好（r=0.999 9）,异鼠李素在0.025~0.075μg（r=0.999 9）范围内线性关系良好;槲皮素和异鼠李素的平均加样回收率分别为99.08%和100.07%;RSD分别为1.36%和0.98%（n=6）。结论 HPLC法可快速准确地测定沙枣中黄酮类物质的含量,为沙枣黄酮类物质的进一步开发提供了可靠的依据。
Objective To determine the content of quercetin and isorhamnetin in Elaeagnus angustifolia by high performance liquid chromatography. Methods The separation was performed on C18 column and the mobile phase was methanol-0. 1% phosphoric acid solution（ 58： 42）,quercetin and isorhanmetin was detected at 370 nm. Results The standard curve was linear within the concentration range of 0. 016 ~ 0. 032 μg for quercetin and 0. 025~ 0. 075 μg for isorhamnetin,respectively. The recovery was 99. 08% for quercetin and 100. 07% for isorhanmetin,respectively. RSD was 1. 36% for quercetin and 0. 98% for isorhanmetin（ n = 6）. Conclusion This study provides a fast and accurate method to determine the content of flavonoids substances in Elaeagnus angustifolia,provides the reliable basis for the further development of flavonoids substances in Elaeagnus angustifolia.
出处
《新疆医科大学学报》
CAS
2015年第12期1510-1511,1516,共3页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区科技厅公益性科研院所基本科研业务资助项目(KY2015133)
关键词
沙枣
高效液相色谱法
槲皮素
异鼠李素
含量测定
Elaeagnus angustifolia L.
HPLC
quercetin
isorhamnetin
determination of content
