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亥茅酚苷对脂多糖诱导巨噬细胞一氧化氮和白介素6表达的影响 被引量:11

Effects of Sec-O-Glucosylhamaudol on expressions of nitric oxide and interleukin-6 in Lipopolysaccharide stimulated murine macrophage
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摘要 目的:探讨亥茅酚苷对脂多糖(LPS)诱导的小鼠巨噬细胞株RAW264.7炎症介质及细胞因子(NO、IL-6)释放的影响。方法:应用LPS(1μg/ml)刺激RAW264.7细胞,采用不同浓度亥茅酚苷(20、40、80μg/ml)干预,Griess试剂法测定NO释放量;酶联免疫吸附试验(ELISA)测定IL-6释放,用免疫印迹法(Western blot)检测细胞一氧化氮合酶(i NOS)的表达,实时荧光定量反转录聚合酶链反应(RT-PCR)技术分析i NOS、IL-6 mRNA的表达。结果:亥茅酚苷各剂量组(20、40、80μg/ml)均能抑制LPS诱导的RAW264.7细胞NO、IL-6的释放(P<0.01),并下调i NOS蛋白、i NOS mRNA、IL-6 mRNA的表达。结论:亥茅酚苷对LPS诱导的巨噬细胞NO、IL-6释放和i NOS表达有抑制作用,具有一定的抗炎活性。 Objective: To investigate the effect of Sec-O-Glucosylhamaudol on inflammatory mediator and cytokine( NO and IL-6) production in Lipopolysaccharide( LPS) stimulated murine macrophage( RAW264. 7). Methods: Macrophages were induced with LPS,and incubated with different concentrations of Sec-O-Glucosylhamaudol( 20,40,80 μg/ml),the quantity of NO production was measured by Griess reagent; the IL-6 production were measured by enzyme linked immunosorbent assay( ELISA),the expression of nitric oxide synthase( iNOS) in cells were detected by Western blot; the expression of iNOS and IL-6 mRNA were analyzed by real-time PCR. Results: Each concentrations of Sec-O-Glucosylhamaudol( 20,40,80 μg/ml) inhibited the production of NO and IL-6 in LPSstimulated RAW264. 7 cells( P〈0. 01). This compound also reduced the mRNA expression of iNOS and IL-6. Conclusion: Sec-O-Glucosylhamaudol exhibited anti-inflammatory activity by inhibited the NO and IL-6 production in LPS stimulated RAW264. 7 cells.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2015年第11期1486-1489,共4页 Chinese Journal of Immunology
关键词 亥茅酚苷 巨噬细胞 炎症 一氧化氮 白介素6 Sec-O-Glucosylhamaudol Macrophage Inflammation Nitric oxide Interleukin-6
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  • 1王成章,张崇禧.防风国内外研究进展[J].人参研究,2008,20(1):35-41. 被引量:32
  • 2高咏莉.生药防风的化学成分与药理作用研究进展[J].山西医科大学学报,2004,35(2):216-218. 被引量:54
  • 3Shen DD, Xie X J, Zhu Z J, et al. Screening active components from Yu-Ping-Feng-San for regulating initiative key factors in allergic sensitization [J]. PloS 0ne,2014,9(9) :1-11.
  • 4Green LC, Wagner DA, Glogowski J, et al. Analysis of nitrate, nitrite, and [ 15N ] nitrate in biological fluids [ J 1. Anal Bioehem, 1982,126( 1 ) :131-138.
  • 5White M. Mediators of inflammation and the inflammatory process [ J ]. J Allergy Clin Immunol, 1999,103 (3 Pt 2 ) : s378-s381.
  • 6Leirisalo-Repo M. The present knowledge of the inflammatory process and the inflammatory mediators [ J ]. Pharmacol Toxicol, 1994,75 (Suppl 2) :1-3.
  • 7Cross RK, Wilson KT. Nitric oxide in inflammatory bowel disease [J]. Inflamm Bowel Dis,2003,9(3) :179-189.
  • 8Nagy G, Koncz A, Telarico T, et al. Central role of nitric oxide in the pathogenesis of rheumatoid arthritis and systemic lupus erythematosus [ J ]. Arthritis Res Ther,2010,12 ( 3 ) : 210 -216.
  • 9陈婧,方建国,吴方建,施春阳,熊苗苗,王文清.鱼腥草抗炎药理作用机制的研究进展[J].中草药,2014,45(2):284-289. 被引量:120
  • 10李霞,于庆海,艾朋,甘艳军,付正武.人工培植牛黄抗炎作用及其机制的初步探讨[J].沈阳药科大学学报,2000,17(6):431-433. 被引量:15

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