摘要
目的:比较染色体核型分析与八探针间期荧光原位杂交(fluorescence in situ hybridization,FISH)两种方法在检测急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)儿童细胞遗传学异常方面的差异。方法:分析39例ALL儿童行染色体核型与八探针间期FISH的结果。结果:39例ALL儿童患者中染色体核型异常阳性率为33.33%,八探针FISH系统检测基因异常阳性率为87.18%,两者间比较差异有统计学意义(P<0.05)。结论:八探针FISH系统检测ALL患者细胞遗传学异常的阳性率高于染色体核型分析的阳性检出率,且方便快捷;八探针FISH系统联合染色体核型分析技术,能明显提高了儿童患者细胞遗传学检测的效率。
Objective：To compare the differences between karyotype analysis and eight -probe fluorescence in situ hybridization （ FISH） in the detection of cytogenetic abnormalities in children with acute lymphoblastic leukemia （ ALL） . Methods：Karyotype analysis and eight-probe FISH were performed for 39 cases of ALL children. Results：The positivity rates of Karyotype analysis （33. 33 %） and eight-probe FISH （87. 18%） were differed significantly in 39 ALL children （ P 〈0. 05）. Conclusion：Eight-probe FISH is more effi-cient and convenient in the detection of cytogenetic abnormalities in ALL children. The two methods can be well complementary in the clinical diagnosis of ALL children.
出处
《包头医学院学报》
CAS
2015年第11期21-22,共2页
Journal of Baotou Medical College
基金
国家自然科学基金项目(81170521)
