摘要
目的建立大鼠全血中3种CYP450探针药物咖啡因（CYP1A2）、咪达唑仑（CYP3A1）和氯唑沙宗（CYP2E1）的HPLC-MS/MS分析方法.方法样品经甲醇蛋白沉淀后,用高效液相色谱-串联质谱仪检测,采用多个特征离子对定性及外标标准曲线法对3种CYP450探针药物定量分析.结果大鼠全血中咖啡因在5-100 ng/m L浓度范围内线性良好（r=0.9999）,咪达唑仑在1-100 ng/m L浓度范围内线性良好（r=1.0000）,氯唑沙宗在1-100 ng/m L浓度范围内线性良好（r=1.0000）,最低定量限分别为1 ng/m L、0.05 ng/m L和0.1 ng/m L;3种CYP450探针药物的提取回收率均高于90%,日内及日间精密度均小于10%.结论该方法灵敏度高、重现性好、操作简便快速,可适用于cocktai L探针药物法评价CYP450酶的活性研究及法医学鉴定.
Objective To develop a rapid and sensitive method for the simultaneous analysis of three cytochrome P450 probe substrates（caffeine、 midazolam and chlorzoxazone） in rat blood by LCESIMS/MS method.Methods The samples were precipitated with methanol and analyzed with HPLCMS/MS method.Multiple characteristic ion pairs were adopted to three cytochrome P450 probe substrates for qualitative analysis and the external standard method was adopted to three cytochrome P450 probe substrates for quantitative analysis.Results The linear ranges were 5∽100 ng/m L for caffeine（r=0.9999）、1∽100 ng/m L for midazolam（r=1.0000）、1∽100 ng/m L for chlorzoxazone（r=1.0000） and their detection limits were 1 ng/m L、0.05 ng/m L and0.1 ng/m L, respectively.The extract recoveries of three cytochrome P450 probe substrates were above 90%,intraand interday precisions were all less than 10%.Conclusion The method is accurate、sensitive、specific and simple and can be used to evaluate CYP450 enzyme activity and forensic medical identification.
出处
《昆明医科大学学报》
CAS
2015年第11期3-7,共5页
Journal of Kunming Medical University
基金
云南省应用基础研究基金资助项目(2013FB118)
昆明医科大学研究生创新项目(2015S07)
