摘要
以苹果、姜、蒜为试材,采用对峙培养法研究了健康苹果果实、姜、蒜内的可培养内生细菌和健康苹果植株根际土壤微生物对苹果轮纹病的拮抗作用。结果表明:根际土壤中分离到可培养细菌9种,姜、蒜、苹果内分别分离到可培养菌株5种、3种和1种;对分离到的微生物进行拮抗性测定,除土2对苹果轮纹病菌菌丝生长无抑制效果外,其余菌株都对苹果轮纹病菌有抑菌效果,且从根际土壤中分离到的微生物对苹果轮纹病菌具有拮抗性的所占比例较大,为44.4%;其次为姜和蒜,所占比例分别为27.8%和16.7%。所有菌株中除蒜12,土1和土5与对照比较抑菌效果差异不显著外,其余菌株抑菌效果差异都显著。所有菌株中抑制效果最好的为姜4,其真菌菌落直径只有(30.78±0.49)mm,其次为土4,真菌菌落直径为(35.12±0.13)mm。
Apple,garlic and ginger were used as test materials,the confrontation culture method was aolopted to study the antagonism effect from culturable endophytic bacteria within healthy apple fruit,ginger,garlic,and the rhizosphere soil microorganisms in healthy apple plant for the apple ring spot.The results showed that,nine kinds of bacteria in rhizosphere soil can be cultured;the number of strains isolated from ginger,garlic and apple was 5,3and 1,respectively.All of the isolated microorganisms except‘soil-2＇obtained from soil had the inhibition ability and isolates from the rhizosphere soil had greater proportion of antagonism,44.4%;followed by ginger and garlic,the proportion was 27.8%and 16.7%.All strains except‘garlic 1 ②＇,‘soil-1＇and ‘soil-5＇had significant difference comparing with CK.The‘ginger 4＇had the best inhibitory effect,the diameter of fungal colonies was only（30.78±0.49）mm,‘soil-4＇was following,fungal colony diameter was（35.12±0.13）mm.
出处
《北方园艺》
CAS
北大核心
2015年第23期115-119,共5页
Northern Horticulture
基金
衡水市科学技术研究与发展计划资助项目(14064)
河北省高等学校青年拔尖人才计划资助项目(BJ201406)
关键词
苹果轮纹病
可培养内生菌
根际土壤
拮抗
apple ring spot
culturable endophytic bacteria
rhizosphere soil
rivalry
