摘要
目的:观察美洲大蠊提取物(extract from Periplaneta americana,EPA)与环磷酰胺(cyclophosphamide,CTX)联合应用对小鼠H22肿瘤生长的影响并探讨其可能的作用机制。方法:采用KM小鼠皮下接种H22肝癌细胞形成实体瘤模型,分别给予高、中、低剂量(50,100,200 mg·kg-1)EPA,阳性药CTX(30 mg·kg-1)及二者联合治疗10 d后,测定各组小鼠瘤重,肝脏、脾脏指数,通过测定肝脏组织中丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)水平检测药物的抗氧化活性,检测血清中肿瘤坏死因子-α(TNF-α)和白细胞介素2(interleukin 2,IL-2)的含量,并计数联合用药组小鼠血浆中白细胞的数量,以此评价EPA与CTX合用的增效减毒作用。结果:与模型组比较,各给药组均能明显抑制H22荷瘤小鼠肿瘤的生长(P<0.05),尤以50 mg·kg-1EPA与CTX联合用药作用最为显著,其抑瘤率达到了61.96%;单用EPA以及EPA与CTX联合应用均能提高肝组织中SOD水平并降低其中的MDA含量,明显增加血清中TNF-α和IL-2的含量(P<0.05,P<0.01);各剂量EPA均能提高荷瘤小鼠的脾脏指数和胸腺指数,而联合用药各组对荷瘤小鼠脏器指数无明显影响,但是可以提高荷瘤小鼠外周血中白细胞(white blood cells,WBC)的数量。结论:EPA与CTX联合应用对H22肝癌小鼠有明显抑瘤作用,其作用的发挥可能与增强免疫、抗氧化作用有关。
Objective： To observe the synergistic antitumor effect and possible mechanism of extract from Periplaneta americana（ EPA） and cyclophosphamide（ CTX） on mice bearing-H22. Method： KM mice were inoculated subcutaneously with H22 cells to form solid tumor models and then were treated for 10 days with high dose,middle dose and low dose（ 50,100,200 mg·kg^- 1） EPA,30 mg·kg^- 1 CTX or received combination therapy respectively. 10 days later,the tumor weight,liver index,and spleen index were measured; and the levels of malondialdehyde（ MDA） and superoxide dismutase（ SOD） in liver tissues were measured to detect the antioxidant activity of drugs; the contents of tumor necrosis factor-α（ TNF-α）,interleukin 2（ IL-2）,and the number of white blood cells were detected to evaluate the synergistic antitumor effect of EPA and CTX. Result：Compared with the model group,all the treatment groups could inhibit the tumor growth（ P 〈0. 05）,most significantly in combination group of 50 mg·kg^- 1and CTX,with the tumor inhibitory rate up to 61. 96%. EPA alone and combination of EPA and CTX could increase SOD level in liver tissues,reduce MDA level,and significantly increase serum TNF-α and IL-2 levels（ P 〈0. 05,P 〈0. 01）. The various doses of EPA could improve the spleen index and thymus index of tumor-bearing mice,but combination groups had no significant effect on the organs of tumor-bearing mice,but could enhance the amount of white blood cells in the peripheral blood.Conclusion： Combined application of EPA and CTX has significant antitumor effect in mice bearing-H22,and the mechanism may be associated with antioxidant activity and enhanced immunity.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第23期130-134,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
云南省昆虫生物医药研发重点实验室开放课题项目(201406-04)
关键词
美洲大蠊
环磷酰胺
H22荷瘤小鼠
白细胞
肿瘤坏死因子Α
白细胞介素2
Periplaneta americana
cyclophosphamide
mice bearing-tumor H22
white blood cell
tumor necrosis factor-α
interleukin-2
