摘要
目的建立16SrRNA基因荧光定量PCR方法检测心血管病患者术后血流感染的诊断技术,以提高临床细菌检测的准确性及敏感性,缩短检测细菌的时间。方法 2010年4月-2012年3月医院对411例术后疑为血流感染的标本进行常规血液培养,同时进行细菌16SrRNA基因的检测,包括DNA提取、设计引物和探针、聚合酶链反应(PCR)扩增及对扩增产物荧光定量检测,比较二者的诊断阳性率、敏感性和特异性,数据采用SPSS11.0软件进行统计分析。结果荧光定量PCR方法检测血流感染的阳性患者182例,阳性率为44.3%,明显高于血液培养的15.6%,差异有统计学意义(P<0.01);若以血液培养阳性和(或)临床诊断血流感染的标准作为对照,PCR的诊断敏感性为87.9%、诊断特异性为90.6%。结论荧光定量16SrRNA基因检测的阳性率远高于血液培养,可为心血管病患者术后血流感染提供早期、敏感的病原学诊断依据。
OBJECTIVE To develop a FQ-PCR method based on 16S rRNA genes for the diagnosis of blood-stream infections after cardiac surgery in order to improve the efficiency and accuracy of bacterial detection. METHODS Totally 411 samples suspected with blood-stream infections were cultured and bacterial 16S rRNA genes were de- tected synchronously by extraction of DNA, primer and probe design,PCR amplification and fluorogenie quantita- tive detection of amplified products. The positive rate, sensitivity and specificity of the two methods were com- pared. RESULTS The positive rate of FQ-PCT was 44.3~ （182 patients） by fluorogenic quantitative PCR, which was significantly higher than 15.6% by blood culture. Taking the criteria of positive blood culture and/or clinical diagnosis of blood-stream infection as control, the sensitivity was 87.9% and the specificity was 90.6% for FQ- PCR. CONCLUSION The positive rate of FQ-PCT was significantly higher than that of blood culture and could be an early and sensitive method in pathogenic diagnosis of blood-stream infections after cardiac surgery.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第23期5290-5292,共3页
Chinese Journal of Nosocomiology
基金
北京市吴阶平医学基金资助项目(2012-BKJ08)
