摘要
目的了解肠杆菌科细菌产超广谱β-内酰胺酶(ESBLs)与Amp C酶基因型特征。方法对临床分离的287株大肠埃希菌和肺炎克雷伯菌筛选产ESBLs/AmpC酶的菌株进行表型确证试验。对常见的SHV、TEM超广谱β-内酰胺酶基因型和DHA-1、ACT-1 AmpC酶基因型进行PCR扩增,并对PCR产物进行琼脂糖凝胶电泳分析。结果表型确证产酶大肠埃希菌SHV、TEM、DHA-1、ACT-1基因的阳性率分别为46.5%、49.5%、50.9%、32.1%,产酶肺炎克雷伯菌SHV、TEM、DHA-1、ACT-1基因的阳性率分别为50.0%、26.7%、62.5%、45.8%。部分分离菌株同时携带2种或以上基因型。结论临床分离的产ESBLs与AmpC酶大肠埃希菌及肺炎克雷伯菌携带多种耐药基因比率较高,临床检出多重耐药细菌逐年升高,临床对产酶的多重耐药菌的防治迫在眉睫。
Objective To investigate the genotypic character of Enterobacteriaceae bacteria producing extended spectrum β-lactamases（ ESBLs） and Amp C β-lactamase. Methods 287 screened strains of Escherichia coli and Klebsiella pneumoniae suspected to produce ESBLs / AmpC were conducted for phenotypic confirmation test. The SHV,TEM in ESBLs-producing isolates and the DHA-1 and ACT-1 AmpC isolates were amplified by PCR and the results were analyzed by agarose gel electrophoresis. Results Phenotypic confirmation test showed that the positive rates of SHV,TEM,DHA-1 and ACT-1 genes were respectively 46. 5%,49. 5%,50. 9%,32. 1% in all the Escherichia coli producing enzyme,and that were respectively50. 0%,26. 7%,62. 5%,45. 8% in all the Klebsiella pneumoniae producing enzyme. Some strains carried more than 2 genotypes. Conclusion The carrying rate of resistance genes of Escherichia coli and Klebsiella pneumoniae producing ESBLs and Amp C β-lactamases is high. The detected multi drug resistant bacteria in clinic increased year by year,and the clinical treatment of the resistant bacteria producing enzyme is extremely urgent.
出处
《中国卫生检验杂志》
CAS
2015年第23期4101-4103,共3页
Chinese Journal of Health Laboratory Technology
